木豆Actin基因片段的克隆及序列分析  

Cloning and Sequence Analysis of Actin Gene Fragment from Cajanus cajan

作　　者：乔光[1,2] 文晓鹏[1] 

机构地区：[1]贵州大学贵州省农业生物工程重点实验室,贵州贵阳550025 [2]贵州大学林学院,贵州贵阳550025

出　　处：《贵州农业科学》2010年第8期5-7,共3页Guizhou Agricultural Sciences

基　　金：国家自然科学基金项目"利用GeneFishing解析VA菌根增强木豆抗旱能力的分子机理"(30860224);贵州省科学技术基金资助项目"在转录水平解析木豆抗旱的分子机理"[黔科合J字(2009)2076]

摘　　要：根据大豆Actin基因的保守序列设计一对引物Primer 1和Pri mer 2,以木豆叶片总RNA为模板,采用RT-PCR的方法扩增出Actin基因片段并克隆到PMD-18T载体。阳性克隆经PCR检测后测序,序列分析结果表明,该片段长302bp,编码100个氨基酸;所得序列与GenBank中注册的其他植物Actin基因核苷酸序列的同源性均在89%以上,其中与大豆的同源性达94%;与氨基酸序列的同源性均在90%以上。A pair of primers,Primer 1 and Primer 2,was designed according to the conserved sequences of the Actin gene from Glycine max.The Actin gene fragment was amplified by RT-PCR based on total RNA of Cajanus cajan leaves and then cloned into PMD-18T vector.The positive clone was identified via PCR and then sequenced.The sequence analysis showed that the Actin gene fragment from Cajanus cajan contains 302 bp and 100 amino acids.The homology of nucleotide sequence and amino acid sequence of the obtained sequence is above 89% and 90% compared with the nucleotide sequence and amino acid sequence of Actin gene of other plants registered in GenBank.The nucleotide sequence homology of the obtained sequence is up to 94% compared with the nucleotide sequence of Actin gene of Glycine max.

关 键 词：木豆 ACTIN基因 克隆 序列分析 

分 类 号：S529[农业科学—作物学]