紫锥菊、黄芪对感染IBDV雏鸡胸腺、小肠中CD4^+、CD8^+ T淋巴细胞动态分布的影响  

Effect of echinacea-astragalus on the dynamic distribution of CD4^+,CD8^+ T lymphocytes in thymus and small intestine of chickens infected with IBDV

作　　者：倪耀娣[1] 卫书鹏[1] 徐建忠[2] 钟秀会[1] 

机构地区：[1]河北农业大学中兽医学院,河北定州073000 [2]河北农业大学动物科技学院,河北保定071001

出　　处：《中国兽医学报》2010年第8期1075-1078,共4页Chinese Journal of Veterinary Science

基　　金：河北省科技攻关计划资助项目(07220401D)

摘　　要：将14日龄雏鸡随机分为3组,A组灌服1 mL的紫锥菊、黄芪合剂,B、C组灌服生理盐水,连续灌服7 d。21日龄时A、B组雏鸡接种传染性法氏囊病病毒(IBDV),用免疫组化法观察雏鸡胸腺、小肠中CD4+、CD8+T淋巴细胞动态分布的变化。结果表明,用药组和攻毒对照组相比较,CD4+T淋巴细胞差异显著(P<0.05),但是CD8+T淋巴细胞差异不显著(P>0.05),与空白对照组相比CD4+淋巴细胞显著增多。胸腺、小肠中24日龄时CD4+、CD8+T细胞数量较其他日龄差异显著(P<0.05)。总试验期胸腺中CD4+、CD8+含量是所测总数的60%。表明紫锥菊、黄芪对机体免疫有显著增强作用,以胸腺中CD4+、CD8+含量多于小肠,能显著降低IBDV对机体造成的损伤。In order to investigate the effect of active constitutents of echinacea、astragalus on immunoloregulation mechanism of IBDV chickens,14-day-old chickens were randomly allocated into 3 groups.Group A was administrated with echinacea-astragalus mixture at a concentration of 1 mL,Group B and C with physiologic saline at the same time for succesive 7 days.The chickens in Group A and B were inoculated with infectious bursal disease virus（IBDV） at 21-day-old,then the dynamic distribution of CD4＋,CD8＋T lymphocytes in thymus and small intestine was detected by immunohistochemical examination.The results showed that,there exists differences in CD4＋ lymphocyte of drug treated group and control group（P0.05）,but no significance of CD8＋ lymphocytes in both groups was obseved（P0.05）.Compared with blank control group,CD4＋ lymphocytes increased significantly（P0.05）.At 24-day-old,CD4＋ and CD8＋T lymphocytes in thymus and small intestine also showed difference（P0.05）.In the whole experimental stage,CD4＋,CD8＋T lymphocytes in thymus account for 60 percent of the examined thymus cells,and are more than those in small intestine.The result indicated that echinacea-astragalus mixture has a function of strengthening the immune function,as well as alleviating the pathological injury induced by IBDV notablely.

关 键 词：紫锥菊 黄芪 胸腺 小肠 CD4+ CD8+ 鸡传染性法氏囊病病毒 

分 类 号：S853.75[农业科学—临床兽医学]