分次加碲法培养高富碲量小球藻及其对藻体抗氧化酶系统的影响  

Culture of High Tellyrium-Enriched Chlorella Vulgaris with a Stepwise Tellurium Addition Method and its Effects on the Antioxidant Enzymes of the Microalgae

作　　者：蒋洁[1] 陈填烽[1] 杨芳[1] 郑文杰[1] 

机构地区：[1]暨南大学化学系

出　　处：《食品研究与开发》2010年第8期163-167,共5页Food Research and Development

基　　金：国家自然科学基金(20771044);广东省科技计划项目(2008A030201020,2007B030703007)

摘　　要：在小球藻（Chlorella vulgaris,C.vulgaris）生长周期的第7、8、93天分次添加无机碲（Na2TeO3）,维持碲剂量（χTe：以单位生物量C.vulgaris的加碲量为碲剂量）为0.25～1.00,进行富碲培养。观察不同剂量处理组中小球藻对无机碲的富集转化及最终生物量、水溶性蛋白含量、抗氧化酶活性的变化情况。结果表明,χTe为1.00时碲产量高达67.79mg/g,其中有机碲产量为18.62mg/g;富碲C.vulgaris的生物量和水溶性蛋白都有明显增加,即控制剂量在1.00以内可维持甚至促进C.vulgaris的生长。分次加碲法是小球藻高值化处理的有效途径。The cultivation of high tellurium（Te）-enriched Chlorella vulgaris（C.vulgaris）was achieved by using a stepwise Te addition method,in which Te was added in the 7th,8th and 9th days of the cell growth cycle,and Te doses（χTe）were kept constant at 0.25-1.00.The changes in biomass,water-soluble protein,antioxidant enzymes,Te accumulation and transformation in C.vulgaris were also investigated.The results indicate that the high Te-enriched C.vulgaris with a total Se content of 67.79mg/g and organic Te content of 18.62mg/g could be obtained with the χTe at 1.00.Moreover,stimulative effects in biomass and water-soluble protein were observed in all treatment groups by comparing with the control groups.In conclusion,the stepwise Te addition method was an effective means to achieve high value application of C.vulgaris.

关 键 词：小球藻 碲 分次添加 吸收代谢 

分 类 号：TQ464.7[化学工程—制药化工]