番茄LeMBF1基因的克隆及其在温敏型长花柱突变体T431中的表达  

Cloning of LeMBF1 Gene and Analysis of Its Role in Temperature Sensitive To Stigma Elongation of Long-stigma Mutant in Tomato

作　　者：燕正民[1] 王勇[1] 

机构地区：[1]东北农业大学园艺学院

出　　处：《沈阳农业大学学报》2010年第3期279-283,共5页Journal of Shenyang Agricultural University

基　　金：黑龙江省青年基金项目(2007Q0535-00);东北农业大学创新团队基金项目(CX7002-4-2);东北农业大学“211”人才专项基金项目(2004)

摘　　要：从番茄长花柱突变体T431的低温差减文库中筛选出了1个编码转录辅激活因子基因的EST,获得全长后命名为LeMBF1。LeMBF1基因包含一个420bp的开放阅读框,编码140个氨基酸,其氨基酸序列与马铃薯的StMBF1相似性最高为97%。半定量RT-PCR结果表明：LeMBF1在常温下于T431的根、茎尖生长点、叶中均有表达,但茎尖生长点中表达量极低;将花芽分化期的T431经低温处理3,6,9d后,LeMBF1在根中的表达量不变;在低温处理初期叶中表达量略有增加,随后降低;低温处理3d在茎尖生长点表达量明显增加,其后逐渐降低,推测LeMBF1可能参与花器官的形态建成,抑制T431的花柱伸长。A transcriptional co-activator coding gene EST was screened from long-Stigma mutant tomato T431 low-temperature subtracted library,and its full-length named LeMBF1.LeMBF1 gene contains a 420bp open reading frame encoding 140 amino acids.We found LeMBF1 amino acid sequence has the highest similarity with potato StMBF1,reaching 97%.By semiquantitative RT-PCR analysis showed that LeMBF1 expresses in the roots,stems and leaves at ordinary temperature,but expression in stems is very low.By low-temperature treatments for 3d,6d and 9d,the expression volume in roots was unchanged,and in leaves by a slight increase in the initial low temperature,and then reduced.But the expression in stems was significantly increased after 3d low temperature treatments,which indicated that LeMBF1 could be involved in floral organ built form,inhibit the stigma elongation of T431.

关 键 词：番茄 MBF1 克隆 低温 基因表达 

分 类 号：S641.2[农业科学—蔬菜学]