H-FABP基因多态性与铬营养对猪肉质脂肪性状的影响  

Effect of H-FABP Gene Polymorphism and Cr on Pork Fat Quality

作　　者：许云贺[1,2] 边连全[1] 苏玉虹[2] 刘显军[1] 王瑞年[1] 杨爽[1] 

机构地区：[1]沈阳农业大学畜牧兽医学院,沈阳110866 [2]辽宁医学院畜牧兽医学院,辽宁锦州121001

出　　处：《沈阳农业大学学报》2010年第3期304-308,共5页Journal of Shenyang Agricultural University

基　　金：“十一五”国家科技支撑计划项目(2007BAD71B01;2007BAD71B003)

摘　　要：将18头约60kg的杜长大三元杂交猪随机分为2个处理组,分别饲喂基础日粮和试验日粮,试验日粮为基础日粮中添加吡啶羧酸铬,铬在日粮中的添加浓度为200μg·kg^-1。在体重大约90kg时屠宰,并且进行基因表达和肉质测定、H-FABP基因分型及其与肉质性状的关联分析。结果表明：（1）H-FABP基因型对肌内脂肪和大理石花纹存在显著影响（p〈0.05）,Dd基因型具有最高的肌内脂肪含量和大理石花纹得分,分别为2.358%和1.919;（2）铬营养素对肌内脂肪含量、大理石花纹和基因表达量有显著的影响（p〈0.05）,与对照组相比分别提高16.49%、19.19%和52.94%;（3）H-FABP基因和铬营养的交互作用对肌内脂肪含量有显著的影响（p〈0.05）,试验组DD基因型肌内脂肪含量为2.537%,与对照组DD基因型肌内脂肪含量相比提高了20.24%,提高幅度最大。试验结果表明,铬能够显著提高肌肉组织H-FABP基因表达量,提高肌内脂肪含量和大理石花纹,在改善肉质的研究中应同时考虑遗传和营养效应。18 DLY pigs（60 kg） were randomly divided into two dietary treatments without or with 200μg·kg^-1 of Cr from chromium picolinate.All pigs were slaughtered when their weights were about 90 kg,and determined H-FABP gene expression and meat quality traits.Then the polymorphism of H-FABP gene was analyzed and an associated analysis was conducted.The results are as follows：（1） H-FABP gene polymorphism has very significant effect on IMF（%） and marble（p〈0.05）,IMF content and marbling score of Dd genotype were the highest,they were 2.358% and 1.919 respectively;（2） Cr had significant effect on intramuscular fat（IMF,%）,marbling score and H-FABP gene expression（p〈0.05）,increased 16.49%,19.19% and 52.94% compared with the control group respectively;（3） The interaction between gene and Cr nutriment had significant effect on IMF（%）,IMF（%） of DD genotype in group 2 was 2.537%,increased 20.24% compared with that in group 1,increased range was the highest.These results suggested that dietary Cr addition may increase H-FABP gene expression level,IMF content and marble.These results suggested that both genetic and nutritional factors should be concerned in the improvement of pork quality.

关 键 词：猪 H-FABP基因 铬 基因表达 肉质 

分 类 号：S828.91[农业科学—畜牧学]