中国大豆花叶病毒HC-Pro基因的克隆与序列分析  

Cloning and Sequence Analysis of HC-Pro Gene of Soybean Mosaic Virus

作　　者：刘宁[1,2] 刘若淼[1] 马莹[1] 王大刚[1] 智海剑[1] 

机构地区：[1]南京农业大学国家大豆改良中心,作物遗传与种质创新国家重点实验室,江苏南京210095 [2]烟台出入境检验检疫局技术中心,山东烟台264000

出　　处：《大豆科学》2010年第4期549-554,共6页Soybean Science

基　　金：国家自然科学基金资助项目(30971815);国家科技支撑计划资助项目(2006BAD01A04);国家高技术研究发展计划资助项目(2006A10A111);农业部大豆产业技术体系(nycytx-04);高等学校创新引智计划资助项目(B08025);转基因生物新品种培育重大专项(2008ZX08004-004)

摘　　要：通过反转录-聚合酶链式反应(RT-PCR),克隆了我国6个大豆花叶病毒(SMV)分离物的HC-Pro基因全长cDNA,并测定了其全序列,探索其与病毒致病性和交叉保护作用的关系。结果表明:HC-Pro基因全长为1371nt,编码的HC-Pro蛋白为457个氨基酸,其中包含与病毒移动有关的CCC基序、与蚜传相关的PTK基序等功能结构域。6个SMV分离物的HC-Pro基因核苷酸序列同源性为89.2%～99.9%,由此推导的氨基酸序列同源性为95.4%～99.8%,其中SMV分离物TS216和4434仅有1个碱基差异。将序列信息与6个SMV分离物在10个鉴别寄主上的症状反应以及分离物间的交叉保护作用进行综合分析发现,HC-Pro基因同源性高的SMV分离物在鉴别寄主上的致病性反应没有明显的相似性,而HC-Pro基因同源性高、亲缘关系近的部分SMV分离物之间交叉保护作用明显。结果证明6个SMV分离物的HC-Pro基因高度同源,同源性高低与其在鉴别寄主上的致病性反应没有明显联系,但同源性高、亲缘关系近的分离物间交叉保护作用可能较强。This study aims to clone and sequence full-length cDNA of HC-Pro gene of six SMV isolates using RT-PCR,to observe the reaction of six SMV isolates to ten hosts,and to discuss the relationships between similarity of the gene sequence and virus virulence,effect of cross-protection.The results showed that full-length cDNA of HC-Pro gene had 1371 nucleotides encoding 457 amino acids.The protein HC-Pro had functional domains such as the CCC motif related to virus movement and the PTK motif related to spread by aphids.Homologies of six SMV isolates nucleotides were 89.2%～99.9% and amino acids were 95.4%～99.8%.There was one different nucleotide in sequence of HC-Pro gene between TS216 and 4434.No certain relationship was found between homology of HC-Pro gene of six SMV isolates and their virulence.While,homology of SMV isolates determined effects of cross-protection to a certain extent.

关 键 词：大豆花叶病毒 HC-PRO基因 基因克隆 序列分析 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]