利用花粉管通道法将抗虫基因(cryV)转入大豆的研究  

Introduction of Anti-pest Gene (cry V) to Soybean via Pollen-Tube Pathway

作　　者：武小霞[1] 刘伟婷[2] 刘琦[3] 李静[1] 马永 李文滨[1] 

机构地区：[1]东北农业大学农学院,大豆生物学省部共建教育部重点实验室,黑龙江哈尔滨150030 [2]黑龙江省农业科学院植物脱毒苗木研究所,黑龙江哈尔滨150086 [3]黑龙江省农业科学院园艺分院,黑龙江哈尔滨150086 [4]七台河市新兴区长兴乡人民政府农业综合服务中心,黑龙江七台河154532

出　　处：《大豆科学》2010年第4期565-568,574,共5页Soybean Science

基　　金：转基因生物新品种培育重大专项资助项目(2008ZX08004-002,2009ZX08009-089B);哈尔滨市科技创新人才研究专项资金资助项目(2008RFQXN013);东北农业大学博士后基金与博士启动基金资助项目(2009RC47)

摘　　要：利用花粉管通道法,在大豆的盛花期将外源抗虫基因(cry V)转入到大豆中,转化成活率达43.88%,成荚率达33.6%。将收获的的种子种植在大豆所温室中,通过卡那霉素筛选、PCR鉴定,Southern-blot检测和RT-PCR检测,共检测出5株阳性植株,确定外源抗虫基因(cry V)已转入到大豆中,转化率达1.8‰。Anti-pest gene（cry V） was introduced to soybean via pollen-tube pathway during blossom period.1211 flowers out of 2760 ones survived 3 days after the operation,the survival rate and podding rate was 43.88%and 33.6%,respectively.The harvested seeds were planted in greenhouse and 5 plants with positive result were obtained after Km screening,PCR identification,southern-blot testing and RT-PCR testing.Results suggestted that the anti-pest gene（cry V） were successfully introduced into those 5 plants and the transformation rate was 1.8‰.

关 键 词：大豆 抗虫基因(cry V) 花粉管通道法 

分 类 号：S565.1[农业科学—作物学]