农杆菌介导大豆遗传转化技术的研究进展  

Approaches to Agrobacterium-mediated Transformation in Soybean

作　　者：于洋[1] 侯文胜[1] 韩天富[1] 

机构地区：[1]中国农业科学院作物科学研究所,国家农作物基因资源与基因改良重大科学工程,北京100081

出　　处：《大豆科学》2010年第4期696-701,共6页Soybean Science

基　　金：转基因生物新品种培育重大专项资助项目(2008ZX08004-001,2008ZX08010-004-004);农业部财政部现代农业产业技术体系建设专项资助项目(nycytx-004)

摘　　要：农杆菌介导是大豆遗传转化的主要手段。该文从受体基因型及外植体选择、菌株筛选及载体改造、标记基因选用和添加剂应用等方面,介绍了近年来对农杆菌介导大豆遗传转化方法所做的改进,分析了存在问题,展望了近期发展方向。Genetic transformation of soybean（Glycine max（L） Merr.） depends mainly on Agrobacterium mediation system.In this review,the authors introduced the modifications of the technical procedures in this system including the selection of genotypes,explants,strains and makers,plasmid reconstruction,and application of additive agents,etc.We analyzed the disadvantages of this system and took an outlook for the improvement of Agrobacterium-mediated transformation system in soybean in the near future as well.

关 键 词：大豆 农杆菌介导 转基因 

分 类 号：S565.1[农业科学—作物学]