RBSDV-S10基因和SCMV-CP基因双价植物表达载体的构建  

Construction of Binary Expression Vector Carrying RBSDV-S10 Gene and SCMV-CP Gene

作　　者：王迅[1] 江蓓蓓[1] 李晓颖[1] 陈妮[1] 公娇芬[1] 竺晓平[1] 

机构地区：[1]山东农业大学植物保护学院,山东泰安271018

出　　处：《山东农业科学》2010年第8期1-4,共4页Shandong Agricultural Sciences

基　　金：中国博士后科学基金(20070411103);山东省博士后创新项目专项基金(200702015)

摘　　要：克隆了水稻黑条矮缩病毒(RBSDV)的外壳蛋白S10基因的片段和甘蔗花叶病毒(SCMV)的外壳蛋白CP基因的片段,将两个片段在pMD18-T Simple Vector上连接起来。获得的S10-CP片段全长为830bp,将其分别以正向和反向插入植物表达载体pMCG161,构建了含两种不同病毒来源基因的RNAi植物表达载体pMCG161-CP10,为进一步转化玉米,探索利用RNAi技术同时防治玉米矮花叶病和玉米粗缩病奠定了基础。Two fragments from RBSDV-S10 and SCMV-CP were cloned by PCR,and inserted into pMD18-T Simple Vector.The combined sequence of S10-CP from two different viral genes was 830 bp.By using the dsRNA binary vector pMCG161 and sequence S10-CP,a new construct of pMCG161-CP10 was obtained containing inverted repeats.This work is valuable for obtaining the anti-virus transgenic maize resisting to two virus diseases（maize rough dwarf disease and maize dwarf mosaic disease）by the way of RNA interference.

关 键 词：玉米 RNAI S10基因 CP基因 

分 类 号：Q782[生物学—分子生物学]