丹参花药愈伤组织诱导因素的研究  

Study of Influential Factors on Anther Callus Induction of Salvia miltiorrhiza

作　　者：解玉丽[1] 王建华[1] 宋振巧[1] 马晓冲[1] 

机构地区：[1]山东农业大学药用植物研究室,山东泰安271018

出　　处：《山东农业科学》2010年第8期5-7,10,共4页Shandong Agricultural Sciences

基　　金：山东省农业良种工程产业化项目[(2007)217-12]

摘　　要：研究了不同激素处理、不同花粉发育时期、不同低温预处理和高温预培养对丹参花药愈伤组织诱导的影响。结果表明:最佳的培养基激素组合为MS+2.0 mg/L6-BA+0.2 mg/L NAA;单核靠边期的花药愈伤组织诱导率最高;4℃低温预处理2 d最有助于愈伤组织发生;高温预培养不利于愈伤组织诱导率的提高。Several factors such as different hormone treatments,pollen developmental stages,low-temperature pretreatment and high-temperature pre-incubation were studied on anther callus induction of Salvia miltiorrhiza.The results showed that 2.0 mg/L 6-BA ＋ 0.2 mg/L NAA was the best hormone combination;the highest anther callus inductivity appeared at the uninucleate eccentric stage;the pretreatment at 4℃ for 2 days was the most conductive to callus occurrence,while pre-incubation at 35℃ was not conductive to the increase of callus induction rate.

关 键 词：丹参 花药培养 愈伤组织 

分 类 号：S567.530.353[农业科学—中草药栽培]