黑树莓茎尖组织培养研究  

Study on Shoot Tip Culture of Black Raspberry

作　　者：孟静静[1] 陈传英[1] 李慧[1] 郭峰[1] 徐平丽[1] 张悦丽[1] 李新国[1] 

机构地区：[1]山东省农业科学院高新技术研究中心,山东济南250100

出　　处：《山东农业科学》2010年第8期8-10,共3页Shandong Agricultural Sciences

基　　金：山东省自然科学基金项目(ZR2009ZD007);博士后创新项目专项资金(200802005);山东省农业科学院青年科研基金

摘　　要：取春季黑树莓的嫩枝条,以茎尖为外植体,MS为基本培养基,添加植物激素6-BA、NAA、GA3筛选培养基,进行茎尖组织培养研究。试验结果表明,诱导茎尖萌发的最佳培养基为MS+0.5 mg/L 6-BA+0.05 mg/L NAA,萌发率高达90%;继代培养最佳培养基为MS+1.0 mg/L 6-BA+0.02 mg/L NAA+6.0 mg/L GA3,繁殖系数达6.0～7.0;生根最佳培养基为MS+0.5 mg/L6-BA+0.1 mg/L NAA,生根率为96%。The shoot tips of black raspberry in spring were used as the explants for tissue culture in the MS basic medium supplemented with 6-BA,NAA and GA3.The best medium for inducing germination of shoot tip was MS＋0.5 mg/L 6-BA＋0.05 mg/L NAA,in which the germination rate was about 90%.The best medium for subculture was MS＋1.0 mg/L 6-BA＋0.02 mg/L NAA＋6.0 mg/L GA3,in which the propagation coefficient was 6.0～7.0.Additionally,the best rooting medium was MS＋0.5 mg/L 6-BA＋0.1mg/L NAA,in which the rooting rate was about 96%.

关 键 词：黑树莓 茎尖 组织培养 

分 类 号：S663.204.3[农业科学—果树学]