红肉桃果实总RNA提取方法的研究  

Study on Methods of RNA Extraction from Blood Flesh Peach Fruit

作　　者：刘莉[1] 段艳欣[1] 樊连梅[1] 刘更森[1] 原永兵[1] 

机构地区：[1]青岛农业大学青岛市现代农业质量与安全工程重点实验室,山东青岛266109

出　　处：《青岛农业大学学报（自然科学版）》2010年第3期205-208,共4页Journal of Qingdao Agricultural University(Natural Science)

基　　金：国家973计划前期研究专项"红肉桃果实花青苷合成机理及其关键基因的分离与鉴定"(2008CB117016)

摘　　要：以红肉桃果实为材料,采用Unizol总RNA提取试剂盒、CTAB法、冷酚法和改良SDS法4种方法分别提取果皮和果肉中总RNA,以期获得提取红肉桃果肉中总RNA的最适方法。比较,发现改良后的SDS法更适合于红肉桃果实总RNA的提取。此方法所得RNA完整性好,纯度和浓度均较高,可用于RT-PCR分析研究及后续实验。The methods of Unizol kit,CTAB and cold phend,and modified SDS were compared to isolate total RNA from blood flesh peach fruit at different coloring stages.The results showed that the modified SDS method was more suitable for RNA extraction from both peel and flesh of blood flesh peach fruit.And the total RNA was proved to be intact,and of high purity and concentration.And hence,the extracted RNA could be used for RT-PCR and further studies.

关 键 词：红肉桃 RNA提取 改良SDS法 

分 类 号：S662.1[农业科学—果树学]