硅藻生长优化富集油脂的促进机理  

Lipid enrichment in diatom cells by growth regulation

作　　者：程军[1] 葛婷婷[1] 冯佳[1] 周俊虎[1] 岑可法[1] 

机构地区：[1]浙江大学能源清洁利用国家重点实验室,浙江杭州310027

出　　处：《能源工程》2010年第4期27-32,共6页Energy Engineering

基　　金：浙江省重点基金资助项目(Z1090532);浙江省重大科技专项资助项目(2008C13023-3);中央高校基本科研业务费专项资助项目(2009QNA4010)

摘　　要：为了促进硅藻生长富集油脂并转化制取生物柴油的能力,以纤细角毛藻和新月菱形藻为代表,研究了硅藻生长条件(pH值、氮浓度、硅浓度、收获时间)对其富集油脂的影响规律和促进机理,发现在缺硅缺氮条件下适当延长收获时间能够显著提高油脂含量,纤细角毛藻的油脂含量和产油能力明显高于新月菱形藻。当培养基中硅浓度(Na2SiO3.9H2O)由200 mg/L降低到0、氮浓度(NaNO3)由12.0 mmol/L降低到0.5 mmol/L、收获时间由8 d延长到32 d时,纤细角毛藻的干燥生物质中油脂含量由13.25%提高到31.74%(达到2.4倍),细胞产油能力达到39.62 mg/L。In order to enrich more lipids in diatoms and convert into more biodiesel fuels,the impacts of cell growth conditions（pH value,nitrogen content,silicon content and harvest time） on lipid enrichment in two diatoms（Chaetoceros gracilis and Nitzschia closterium） were studied.It was found that nitrogen deprivation,silicon deprivation and longer harvest time are beneficial to increase lipid contents in two diatoms,while chaetoceros gracilis has a higher lipid content than nitzschia closterium.The lipid content in chaetoceros gracilis increases from 13.25% to 31.74%（2.4 times） with a lipid yield of 39.62 mg/L,when the silicon（Na2SiO3·9H2O） content decreases from 200 mg/L to 0,nitrogen（NaNO3） content decreases from 12.0 mmol/L to 0.5 mmol/L,and harvest time increases from 8 to 32 days.

关 键 词：硅藻 油脂 生长速率 纤细角毛藻 新月菱形藻 

分 类 号：TK6[动力工程及工程热物理—生物能]