对生玉米5-氨基酮戊酸脱水酶的简单快速纯化  

A Simple Rapid Purification Scheme

作　　者：项艳[1] 范军[1] 朱苏文[1] 李纯[1] 程备久[1] 

机构地区：[1]安徽农业大学

出　　处：《安徽大学学报（自然科学版）》1999年第2期107-110,共4页Journal of Anhui University(Natural Science Edition)

摘　　要：以聚乙二醇６０００分级沉淀、ＤＥＡＥ－ＳｅｐｈａｒｏｓｅＣＬ－６Ｂ，ＰｈｅｎｙｌＳｅｐｈａｒｏｓｅ４Ｂ层析和制备电泳初步纯化了对生玉米的５－氨基酮戊酸脱水酶，它在ｐＨ８．５时比活为３１Ｕ／ｍｇ蛋白，酶纯化了１１２倍，得率为１２％，其亚基分子量为４１ＫＤ，它可以用于酶促合成胆色素原。aminolevulinate dehydratase(ALAD) was purified simply and rapidly from oppsitefolic maize leaves. The procedure contained centrifuge extract,PEG6000 3%-13% fractionation,DEAE Sepharose CL-6B and phenyl Sepharose $B chromatography respectively,and preparative polyacrylamide gel electrophoresis. After it was purified 112 fold and a yield for 12%, ALAD showed a specific activity of 31U/mg protein at pH 8.5. The purified enzyme is not only used for synthesizing PBG but also for couple enzymatic assay of PBGD. It subunit is about 41KD.

关 键 词：氨基酮戊酸 脱水酶 胆色素原脱氨酶 对生玉米 

分 类 号：S513.01[农业科学—作物学] Q949.714.2[生物学—植物学]