AFLP的一种改进方法  

An Improvemental Method of AFLP

作　　者：万春玲[1] 谭远德[1] 

机构地区：[1]南京师范大学生物系

出　　处：《南京师大学报（自然科学版）》1999年第2期88-91,96,共5页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：江苏省教委自然科学基金;南京师范大学引进人才基金

摘　　要：扩增片段长度多态性（ＡｍｐｌｉｆｉｅｄＦｒａｇｍｅｎｔＬｅｎｇｔｈＰｏｌｙｍｏｒｐｈｉｓｍ）技术是一种兼具稳定性及多态检出率高的ＤＮＡ指纹分析技术，具有广泛应用前景，但该技术费用高、操作较复杂．本文对ＡＦＬＰ技术从限制性内切酶水解、扩增条件、检测方法三方面作了一些改进，建立了一套稳定的和简便易行的实验条件，同时用这种改进的方法对家蚕基因组ＤＮＡ的多态性进行了初步研究，得到了良好的ＤＮＡ的多态性检测效果，不仅分辨力较高。Amplified Fragment Length Polymorphism (AFLP),a new DNA fingerprinting technique,is a reliable and powerful approach in detecting polymorphism loci,but more expensive for a general laboratory and more complex in perform.The approach of digestion of DNA,PCR conditions and detection method of PCR porducts were improved in this paper.The results indicated that our method improves the efficiency of detecting polymorphism loci.The method was applied to research silkworm genomic DNA and the result shew that the electrophoresis bands were clear and stable.

关 键 词：分子标记 AFLP 多态性检测 DNA 指纹分析 

分 类 号：Q523.03[生物学—生物化学] Q78