支气管哮喘患者外周血Th17、CD4^+CD25^+Treg细胞表达特征  

The prevalence of blood Th17 and CD4^+CD25^+Treg cells in patients with bronchial asthma

作　　者：施宇衡[1] 时国朝[1] 万欢英[1] 蒋黎华[2] 艾香艳[1] 朱海星[1] 汤葳[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院呼吸科,上海200025 [2]上海交通大学医学院上海市免疫学研究所,上海200025

出　　处：《中国免疫学杂志》2010年第8期740-743,共4页Chinese Journal of Immunology

基　　金：上海市科委基础研究重点项目基金资助(No.08JC1415200)

摘　　要：目的:探讨外周血Th17和CD4+CD25+调节性T细胞(Treg)在支气管哮喘患者中的表达特征。方法:41例慢性持续期哮喘患者,分为间歇-轻度组(n=23)和中重度组(n=18),行肺功能检查和哮喘控制问卷(ACQ)调查,20例正常人作为对照。通过流式细胞术检测外周血Th17和CD4+CD25+Treg细胞的比例。ELISA检测血浆以及植物血凝素刺激24小时后外周血单个核细胞(PBMC)上清液中的IL-17、IL-10、TGF-β水平。结果:中重度哮喘组外周血Th17细胞比例及血浆IL-17水平高于间歇-轻度哮喘和正常人组,而外周血CD4+CD25+Foxp3+Treg细胞比例及血浆IL-10、TGF-β水平则降低。中重度哮喘组PBMC上清液中IL-17水平增高。哮喘患者FEV1(%预计值)与Th17细胞及血浆IL-17表达成负相关,与CD4+CD25+Treg表达成正相关。ACQ平均得分与Th17细胞和血浆IL-17表达成正相关,与外周血CD4+CD25+Treg表达成负相关。结论:中重度哮喘中外周血Th17细胞应答增强,而CD4+CD25+Treg细胞缺乏,哮喘的严重程度及症状控制与外周血Th17/Treg免疫应答失衡密切相关。Objective：To investigate the prevalence of blood Th17 and CD4^＋CD25^＋regulatory T cells （Treg） in patients with bronchial asthma.Methods：41 patients with chronic persistent asthma were divided into intermittent-mild subgroup （n=23） and moderate-severe subgroup （n=18）,lung function was tested and Asthma Control Questionnaire was recorded.20 healthy donors were enrolled as control.Proportions of blood Th17 and CD4^＋CD25^＋Treg cells were determined by flow cytometry.The level of plasma IL-17,IL-10 and TGF-β,as well as the concentrations of IL-17,IL-10 and TGF-β in culture supernatant of peripheral blood mononuelear cells which stimulated by Phytohenmagglutinin,measured by ELISA.Results：In patients with moderate to severe asthma,the proportions of blood Th17 cells and concentration of plasma IL-17 were increased,but CD4^＋CD25^＋Foxp^3＋Treg cells and plasma IL-10 and TGF-β levels were decreased.The concentrations of IL-17 were increased in culture supernatant of moderate to severe subgroup.FEV1 （%predicted） in patients with asthma was negatively correlated to the population of Th17 cells and plasma IL-17 level,whereas positive correlation to frequencies of CD4^＋CD25^＋Treg cells.The mean score of ACQ in asthma was positively correlated to the population of Th17 cells and plasma IL-17 level,with negative correlation to frequencies of CD4^＋CD25^＋Treg cells.Conclusion：The increased response of Th17 cells and deficiency of CD4^＋CD25^＋Treg cells are presented in moderate to severe asthma.The severity and control of asthma may be affected by the imbalance of Th17 and CD4^＋CD25^＋Treg cells response.

关 键 词：支气管哮喘 TH17细胞 调节性T细胞 IL-17 IL-10 TGF-β 

分 类 号：R562.25[医药卫生—呼吸系统]