阿奇霉素对生物被膜菌产AmpC酶、超广谱β-内酰胺酶的影响  

Effect of azithromycin on the AmpC and ESBLs produced in the biofilm of Escherichia coli

作　　者：李乃静[1] 李岩[1] 何平[1] 李胜岐[1] 

机构地区：[1]中国医科大学附属盛京医院,辽宁沈阳110004

出　　处：《沈阳药科大学学报》2010年第8期665-668,共4页Journal of Shenyang Pharmaceutical University

基　　金：辽宁省自然科学基金资助项目(L008225021-2)

摘　　要：目的探讨生物被膜(biofilm,BF)菌的耐药机制及阿奇霉素对BF菌产AmpC酶、超广谱β-内酰胺酶(extended-spectrumβ-lactamases,ESBLS)的影响。方法应用改进的平板培养法建立大肠埃希菌BF模型,扫描电镜鉴定细菌BF。采用改良三维试验法检测ESBLS及AmpC酶。结果浮游大肠埃希菌(A组)单产AmpC,单产ESBLS酶及同时产ESBLS和AmpC酶的菌株分别占总菌株的5.0%(2/40)、20.0%(8/40)、5.0%(2/40);BF大肠埃希菌(B组)产酶菌株分别占总菌株的15.0%(6/40)、47.5%(19/40)、22.5%(9/40);阿奇霉素诱导BF菌(C组)产酶菌株分别占总菌株的7.5%(3/40)、25%(10/40)、12.5%(5/40)。对A组和B组、B组和C组的检出率两两分别进行χ2检验,结果均为P<0.05。结论阿奇霉素能够明显抑制BF大肠埃希菌产生ESBLS及AmpC酶。Objective To evaluate the mechanism that bacteria growing in biofilm always resist to antimicrobial agents,and investigate the effect of azithromycin on the AmpC and ESBLs produced in the biofilm.Methods Model of Escherichia coli bacterial biofilm was built up with the modified flat-board method and identified with confocal scanning laser microscopy.ESBLs and AmpC were detected by improved three dimensional test.Results The detection rates of AmpC,ESBLs and AmpC＋ESBLs in isolated Escherichia coli（group A） were 5.0%（2/40）,20.0%（8/40）,5.0%（2/40）,respectively;The detection rates of AmpC,ESBLs and AmpC＋ESBLs in BF Escherichia coli（group B）were 15.0%（6/40）,47.5%（19/40）,22.5%（9/40）,respectively;The detection rates of AmpC,ESBLs and AmpC＋ESBLs in BF Escherichia coli treated with azithromycin（group C）were 7.5%（3/40）,25%（10/40）,12.5%（5/40）,respectively.Conclusions Azithromycin can inhibit the formation of the AmpC and ESBLs produced in the biofilm.

关 键 词：大肠埃希菌 生物被膜 超广谱Β-内酰胺酶 AMPC酶 阿奇霉素 

分 类 号：R378[医药卫生—病原生物学]