微载体培养PK-15细胞试验条件的优化  

Optimization of Condition for Culture of PK-15 Cell by Microcarrier

作　　者：何锡忠[1] 李春华[1] 倪建平 蒋风英[1] 邹勇[1] 

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海201106 [2]上海佳牧生物制品有限公司,上海201106

出　　处：《动物医学进展》2010年第8期20-23,共4页Progress In Veterinary Medicine

基　　金：上海市科委科技发展基金项目(04391930)

摘　　要：为了优化PK-15细胞在微载体上的生长条件,在微载体浓度5 mg/mL、低糖DMEM+100 mL/L NBS培养液条件下,观察4.5×104cells/mL和8.7×104cells/mL的细胞接种密度对细胞生长的影响;在低糖DMEM+100 mL/L NBS培养液、接种密度5×104cells/mg条件下,观察3、5、10 mg/mL微载体浓度对细胞生长的影响;在3 mg/mL微载体浓度条件下、接种密度25×104cells/mL,观察MEM、高糖DMEM和低糖DMEM培养基对细胞生长的影响。结果表明,以微载体接种细胞密度4.5×104cells/mg、微载体浓度5 mg/mL在低糖DMEM培养基中培养方式效果最为理想。优化的培养工艺适用于PK-15细胞的生产。The aim of this study is to optimize the cultural condition of PK-15 cell by microcarrier system.On the condition of 5 mg/mL microcarrier concentration and low sugar DMEM containing 100 mL/L NBS as culture fluid,the effects on PK-15 cell growth at 4.5×104 cells/mL and 8.7×104 cells/mL inoculation density were observed.On the condition of low sugar DMEM containing 100 mL/L NBS as culture fluid and the 5×104cells/mg inoculation density,the effects on PK-15 cell growth at 3,5,10 mg/mL microcarrier concentration were observed.On the condition of 3mg/mL microcarrier concentration and the 25×104 cells/mL inoculations density,the effects on PK-15 cell growth by using MEM,high sugar DMEM or low sugar DMEM as culture fluid were observed.The results showed that the optimal culture conditions were 4.5×104 cells/mg inoculation density,5 mg/mL microcarrier concentration,and low sugar DMEM as culture fluid.Optimal cultural technology could apply for the production of PK-15 cell.

关 键 词：微载体培养 PK-15细胞 培养条件 

分 类 号：Q813.11[生物学—生物工程]