猪脑心肌炎病毒GXLC株VP1基因的克隆与序列分析  

Cloning and Sequence Analysis of VP1 Gene of Encephalomyocarditis Virus GXLC Strain Isolated from Swine

作　　者：陈进喜 施开创[2] 屈素洁[2] 郑敏[2] 李向涛[3] 陈宏备[3] 许瑞胜[3] 

机构地区：[1]钦州市动物疫病预防控制中心,广西钦州535000 [2]广西动物疫病预防控制中心,广西南宁530001 [3]广西大学动物科学技术学院,广西南宁530005

出　　处：《动物医学进展》2010年第8期45-50,共6页Progress In Veterinary Medicine

基　　金：广西科学基金项目(桂科青0728047);广西科技创新能力与条件建设基金项目(08-05-01D)

摘　　要：应用RT-PCR方法扩增猪脑心肌炎病毒GXLC株的VP1基因,扩增产物克隆入pMD18-T载体后进行测序,对获得的VP1基因序列进行分析。序列分析表明,GXLC株VP1基因长度为831 bp,编码277 aa,含有2个潜在的N-糖基化基序和9个抗原表位。同源性分析表明,GXLC株与国内外其他26个EMCV分离株VP1基因核苷酸序列的同源性在78.6%～99.6%之间、氨基酸序列的同源性在95.1%～99.3%之间。遗传进化分析表明,基于VP1基因序列绘制的系统进化树可以将所有EMCV分离株分成两个群,即Ⅰ群和Ⅱ群,Ⅰ群可再细分为Ⅰa亚群和Ⅰb亚群,其中猪源、鼠源EMCV在Ⅰ群和Ⅱ群中均有分布,人源EMCV则分布在Ⅱ群;GXLC株与其他中国分离株均属于Ⅰa亚群。The structural protein VP1 gene of encephalomyocarditis virus（EMCV） GXLC strain was amplified by RT-PCR,cloned into a pMD 18-T vector,sequenced and analyzed.The sequence analysis showed that the VP1 gene of GXLC strain consisted of 831 nucleotides encoding 277 amino acides and contained 2 potential N-glycosylation motifs and 9 epitopes.The homology analysis indicated the homology of GXLC strain and other EMCV strains available in GenBank were from 78.6%-99.6% and from 95.1%-99.3% based on the nucleotide and deduced amino acide sequences,respectively.The phylogenetic tree based on the VP1 gene sequences revealed that all the EMCV strains could be divided into two groups： group Ⅰ and group Ⅱ,and group Ⅰ could be subdivided into subgroup Ⅰa and subgroup Ⅰb.The strains from pig and mouse distributed in group Ⅰ and group Ⅱ,while the strains from human distributed in group Ⅱ.GXLC strain,together with other EMCV isolates from China,belonged to subgroup Ⅰa.

关 键 词：猪 脑心肌炎病毒 VP1基因 分子特征 

分 类 号：S852.659.6[农业科学—基础兽医学] Q786[农业科学—兽医学]