大相岭山系大熊猫数量及遗传多样性的DNA指纹检测  

THE NUMBERS AND GENETIC DIVERSITY OF GIANT PANDA IN DA XIANGLING MOUNTAIN BY DNA FINGER PRINTING 

作　　者：方盛国[1,2,3,4,5] 冯文和[1,2,3,4,5] 张安居 李光汉[1,2,3,4,5] 余健秋 张志和[1,2,3,4,5] 何光昕 陈红卫[1,2,3,4,5] 郑明全[1,2,3,4,5] 

机构地区：[1]浙江大学生命工程学院 [2]四川大学生物系 [3]成都大熊猫繁育研究基地 [4]成都动物园 [5]瓦山森林公园管理科

出　　处：《四川大学学报（自然科学版）》1999年第3期627-630,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：成都大熊猫繁育研究基金

摘　　要：大熊猫分布在秦岭、岷山、邛崃山、凉山、小相岭和大相岭等六大山系，前５大山系的大熊猫种群数量均有报道［１，２］．１９４８年大相岭山系峨眉山的最后一只大熊猫消失后，对大相岭山系大熊猫数量知之甚少．我们于１９９８年４月中旬，从洪雅县的炳灵乡出发，在白沙河两...Abstract In April 1998,the authors used the road way method to investigate the Wa Mountain in Da Xiangling Mountains and reached more than 1800 meters above sea level.The authors got 22 excrement samples of Giant Panda and collected DNA to check the DNA fingerdrint and analyse the PCR.1.There are 6 Giant Pandas in Da Xingling Mountain 3 female and 3 male and 2 families.In Liuping Dagang,the mothr has been beed,only the father and his one daughters.2.DNA fingerprint method is used to investigate the quantity,the family,the sexual rate and the genetec diversity of Giant Pandas in wild field.It is useful and briefly.3.Among the 6 Mountains where Giant Pandas are distributed,the quantity in Wa Mountain is the most least.At there,the genetic diversity is very poor.The mean allelic frequenay is only 0.5422,mean heterozygous ratio is 45.78% and is the most lowest among 6 mountains,mean similarity coefficient is high to 0.7881,exceeds the maximum of family animals.4.It is extremely urgent to save the Giant Pand in Da Xiangling Mountain.

关 键 词：大熊猫 遗传多样性 种群数量 DNA指纹图 检测 

分 类 号：Q959.838[生物学—动物学]