社区获得性呼吸道感染中肺炎支原体检测方法比较  

The comparison of Mycoplasma pneumonia detecting method in community-acquired respiratory tract infection

作　　者：潘明安[1] 张天托[1] 朱家馨[1] 黄静[1] 刘慧[1] 

机构地区：[1]中山大学附属第三医院呼吸内科,广东广州510630

出　　处：《中国医药导报》2010年第25期5-6,共2页China Medical Herald

摘　　要：目的:比较3种检测肺炎支原体(Mp)的方法,寻求更加适合临床检测要求的检测手段。方法:选择148例社区获得性呼吸道感染患者,留取痰或者咽拭子、血清标本,分别经培养、培养-PCR和血清IgM抗体检测,对检测结果进行分析。结果:3种方法检测Mp的阳性率分别为2.70%、6.76%和11.49%,经统计学检验,培养法与血清学阳性率、培养-PCR法与培养法阳性率间差异均有统计学意义(均P<0.01),培养-PCR法与血清学阳性率间差异无统计学意义(P>0.05)。结论:培养-PCR法和血清学检测是肺炎支原体病原学诊断的有效手段,二者联合使用对于提高肺炎支原体感染诊断有重要帮助。Objective： To compare three methods for detecting the Mycoplasma pneumonia, to explore a method better fitting the clinical demands. Methods： 148 patients with community-acquired respiratory tract infection were selected. Their sputum samples, throat swabs and blood serums were assembled to detect Mp-IgM antibodies through methods of cultivation, culture-PCR and serological test. The results were analyzed. Results： The positive rates of three methods for detecting Mp were 2,70%, 6,76% and 1 L49% respectively, there were statistical differences between cultivation and serological test, as well as euhure-PCR and cultivation （all P〈0.01）. There were no statistical differences between culture-PCR and serological test （P〉0.05）. Conclusion： Cuhure-PCR and serological test are both effective methods for detecting Myeoplasma pneumonia. Combination of the two methods can significantly improve the diagnosis of Mycoplasma pneumonia.

关 键 词：肺炎支原体 培养法 培养-PCR法 血清抗体法 

分 类 号：R56[医药卫生—呼吸系统]