毛细管电泳在线电推扫测定蔬菜中3种氯化烟碱类农药残留  

Determination of Three Chloronicotinyl Insecticide Residues by Capillary Electrophoresis with Sweeping

作　　者：孙娟[1] 陈冠华[1] 王坤[1] 董敏[1] 代永佳[1] 

机构地区：[1]江苏大学食品与生物工程学院,镇江212013

出　　处：《分析化学》2010年第8期1151-1155,共5页Chinese Journal of Analytical Chemistry

基　　金：江苏大学科研基金(No.08JDG001)资助项目

摘　　要：以采用电动进样模式的毛细管电泳在线电推扫技术建立了测定蔬菜中吡虫啉、啶虫脒和噻虫嗪残留的新方法。研究了缓冲液浓度、pH值、十二烷基硫酸钠浓度、分离电压和进样量对峰高的影响,优化后缓冲液为40mmol/L硼酸盐、60mmol/L十二烷基硫酸钠、pH7.8,进样电压10kV,进样时间120s,分离电压25kV。在此条件下,吡虫啉、啶虫脒和噻虫嗪的富集倍数可达626,788和443倍;检出限分别为5.6,2.9和2.5μg/kg;相对标准偏差为1.65%,1.92%和0.96%;平均加标回收率为87.4%～102.5%,95.3%～103.1%和78.7%～93.9%。本方法可满足相关标准中的残留限量要求,已用于蔬菜中这3种农药残留的检测。A new assay was developed by capillary electrophoresis in electrokinetic injection mode and sweeping for determining the residues of acetamiprid,imidacloprid and thiamethoxam in vegetables. The effects of the parameters such as buffer concentration,pH,sodium dodecyl sulfate concentration,separating voltage and sample size,on the peak height of analytes were studied. The optimum buffer composition is 40 mmol/L borate and 60 mmol/L sodium dodecyl sulfate at pH 7.8. Injection voltage is 10 kV and injection time 120 s. Separation voltage is 25 kV. Under these conditions,peak enrichment factor is 626,788 and 443; detection limit is 5.6,2.9 and 2.5 g/kg; RSD is 1.65%,1.92% and 0.96%; average recoveries of spiked sample is 87.4% 102.5%,95.3% 103.1% and 78.7% 93.9% for acetamiprid,imidaclorid and thiamethoxam,respectively. The assay can meet the requirement of maximum residue limits,and has been applied for determining the residues of these three pesticides in vegetables.

关 键 词：吡虫啉 啶虫脒 噻虫嗪 电推扫 胶束电动毛细管色谱 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]