IBV广西分离株GX-YL5在鸡体内动态分布和排毒规律及组织嗜性研究  

Distribution Dynamics,Virus Shedding and Tissue Tropism of Infectious Bronchitis Virus Guangxi Isolate GX-YL5 in Chicks

作　　者：磨美兰[1] 陈秋英[1] 韦英益[1] 朗亚辉[1] 黄柏成[1] 韦子才[1] 李孟[1] 韦平[1] 韦天超[1] 

机构地区：[1]广西大学动物科学技术学院,广西南宁530005

出　　处：《中国家禽》2010年第15期11-14,共4页China Poultry

基　　金：国家自然科学基金(30700599);广西自然科学基金(桂科青0728003;桂科自0991044);广西科技攻关项目(0993009-2);广西高等学校优秀人才资助项目(桂教人才0802)

摘　　要：鸡传染性支气管炎病毒(IBV)广西分离株GX-YL5通过滴鼻点眼人工感染14日龄健康非免疫雏鸡,对接毒后1、3、5、7、10、14、21、28、35和42 d试验鸡的气管、肺脏、肾脏、胸腺、腺胃、盲肠扁桃体和肝脏等组织及泄殖腔棉拭子进行IBV的反转录巢式PCR检测,同时对接毒后不同时间的气管、肺脏、肾脏、胸腺、腺胃、法氏囊、脾脏和肝脏进行组织病理学观察。结果表明接毒后的3～21 d,气管、肺脏、肾脏、胸腺、腺胃、盲肠扁桃体和肝脏的病毒检测均为阳性,接毒后42 d肾脏和气管检测结果仍为阳性;接毒后3～42 d泄殖腔棉拭子检测结果为阳性。组织病理学观察见到气管黏液,气管和细支气管纤毛脱落,炎症细胞浸润,肺充血,淋巴细胞浸润,肾脏间质性肾炎以及法氏囊水肿。研究结果表明,GX-YL5株对雏鸡具有广泛的组织嗜性,主要脏器带毒时间21 d或更长,泄殖腔排毒持续至少39 d。Fourteen-day-age healthy and non-vaccinated chicks were inoculated with Guangxi infectious bronchitis virus （IBV）isolate GX-YL5. Trachea,lung,kidney,thymus,proventriculus,ceeal tonsil, liver and eloacal swabs collected at 1,3,7,10,14,21,28,35,42 days postinfection （DPI）were detected by RT-Nested-PCR. The histopathological lesions of traehea,lung,kidney,thymus,proventriculus,bursa,spleen and liver collected at 1,3,7,10,14,21,28,35,42 DPI were observed. The results showed that lung,trachea,kidney,thymus,proventriculus,cecal tonsil and liver were positive during 3 to 21 DPI. Trachea and kidney were still positive at 42 DPI. All the cloacal swabs samples were positive during 3 to 42 DPI. The histopathological lesions of inoculated chicks included the production of trachea mucus,loss of tracheal and bronchiole cilia,infiltration of inflammation cells,pulmonary congestion,interstitial nephritis and bursa edema. The present study indicated that IBV Guangxi isolate GX-YL5 had extensive tissue tropism,the inoculated chicks carried IBV for 21 days or longer time and shed IBV for at least 39 days.

关 键 词：传染性支气管炎病毒 动态分布 排毒规律 组织嗜性 

分 类 号：S858.31[农业科学—临床兽医学]