利用PCR-DGGE技术分析猪粪堆肥细菌群落结构  

Bacterial Community Structure in Swine Manure Composting by PCR-denaturing Gradient Gel Electrophoresis Technology

作　　者：郭艳[1,2] 张进良[1] 邓昌彦[2] 朱能武[3] 

机构地区：[1]商丘师范学院生命科学系,河南商丘476000 [2]华中农业大学动物科技学院,武汉430070 [3]华南理工大学环境科学与工程学院污染控制与生态修复广东省高校重点实验室,广州510006

出　　处：《河南师范大学学报（自然科学版）》2010年第5期159-162,共4页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家自然科学基金(30471271);国家科技支撑计划(2006BAD14B05-2)

摘　　要：采用PCR-DGGE技术对猪粪堆肥升温期、高温期和降温期上、中、下3层共9个堆肥样品的细菌群落结构演替及其多样性进行了分析.结果表明,不同时期、不同高度层堆肥样品中有着比较丰富的细菌多样性,同时存在着明显的优势种群结构变化.因此,不同时期不同高度层堆肥微生物所表现出的多样性,直接为我们提供了＂梯度效应＂存在的生物学证据,为诠释和控制＂梯度效应＂提供了理论依据.The dynamic change and diversity of bacterial community in the 9 samples,on the superstratum,middle-level and substratum swine manure composting samples during the initial stage,sustained high temperature stage and gradual cooling stage,respectively,are taken and analysed by using PCR-DGGE technique.The results show that the samples of different stages and different locations contain relatively abundant bacterial diversity,and presented changes of the dominant species.It are suggested that the microorganism community diversity among the different location samples in the different stages provide the biology evidence of the phenomenon ＂gradient effect＂,which provides the theoretical basis to annotate and control the ＂gradient effect＂.

关 键 词：PCR-DGGE 猪粪堆肥 细菌多样性 梯度效应 

分 类 号：Q938.1[生物学—微生物学]