蓖麻纯合骨干亲本的亲缘关系分析  

Analysis on genetic relationship among castor-oil plant materials

作　　者：毕川[1] 刘思洁[1] 余晓琴[1] 刘世勇[1] 陆建农[1] 殷学贵[1] 

机构地区：[1]广东海洋大学农学院,广东湛江524088

出　　处：《广东农业科学》2010年第8期191-193,共3页Guangdong Agricultural Sciences

基　　金：广东省教育厅项目(0909127);广东海洋大学自然科学项目(0812039)

摘　　要：调查了24份蓖麻纯合骨干亲本材料的30个重要性状,利用19对SRAP(相关序列扩增多态性)多态性引物对其基因组DNA进行了多态性检测,整合表型数据和分子标记数据进行聚类分析,确定了24份材料之间的亲缘关系。研究结果表明:大部分栽培材料在遗传距离10.11处聚成一类,而大部分野生材料在此类之外且比较分散。说明现有栽培材料遗传基础比较狭窄(遗传距离7.49～10.56),野生材料亲本遗传多样性比较丰富(遗传距离10.64～17.36),野生材料和栽培材料之间总体上存在着较大的遗传差异。加大华南野生蓖麻资源的保护和研究力度,并用于拓宽亲本遗传基础是蓖麻育种的当务之急。还提出了适用于SRAP-PCR扩增的蓖麻高质量DNA提取方法。30 characters were investigateded in 24 castor backbone parent materials and their genomic DNA Were amplified with 19 pairs of polymorphic SRAP primers,and then the relationship among those materials was analysed with clustering after integrating the phenotypic data and molecular marker data.The results indicated that the genetic diversity was poor among modern-variety parents （ the genetic distances ranged from 7.49 to 10.56 ） and rich among wild parents （the genetic distances ranged from 10.64 to 17.36）,there was distinct genetic difference between these two type of parents,which suggested that it was urgent to enrich the genetic diversity utilizing the wild gernplasm from south China for raising the castor breeding level.Meanwhile,one method was proposed for extracting high-quality genomic DNA suitable for SRAP-PCR amplification.

关 键 词：蓖麻 SRAP 亲缘关系 聚类分析 

分 类 号：Q348[生物学—遗传学] S565.6[农业科学—作物学]