Hypoxia-ischemia altered expression of glutamate transporter EAAT1 in neonatal rat brain  

Hypoxia ischemia altered expression of glutamate transporter EAAT1 in neonatal rat brain 1

作　　者：陶锋[1,2] 张玲妹[1,2] 孙凤艳 

机构地区：[1]上海医科大学神经生物学教研室医学神经生物学国家重点实验室 [2]上海生命科学研究中心

出　　处：《中国药理学报》1999年第2期107-111,共5页Acta Pharmacologica Sinica

摘　　要：目的：观察缺氧缺血后新生大鼠脑内谷氨酸载体ＥＡＡＴ表达的改变．方法：用免疫组织化学方法检测ＥＡＡＴ的表达水平．结果：在假手术对照组，ＥＡＡＴ１仅少量表达在大脑皮层［每脑片（３６±１０）个细胞］；缺氧缺血后２４ｈ和４８ｈ，ＥＡＡＴ１在大脑皮层的表达明显增加，分别为每脑片（３１４±１６２）个细胞和（４３１±１４９）个细胞；缺氧缺血后７２ｈ，ＥＡＡＴ１在大脑皮层的表达恢复到对照组水平［每脑片（５２±８）个细胞］．缺氧缺血后ＥＡＡＴ１在颈总动脉结扎同侧大脑皮层的表达明显高于对侧皮层．结论：缺氧缺血后ＥＡＡＴ１在新生大鼠大脑皮层的表达随时间而改变；且主要定位于颈总动脉结扎同侧皮层上．AIM: To observe altered expression of glutamate transporter EAAT1 after hypoxia ischemia (H I) in newborn rat brain. METHODS: Expression levels of EAAT1 were detected with immuno￣histo￣chemistry method. RESULTS: EAAT1 was a little expressed in cerebral cortex at sham operated group [(36±10) cells/slice]. Its expression in cerebral cortex increased at 24 h and 48 h following H I [(314±162) cells/slice and (431±149) cells/slice, respectively], and recovered to control level at 72 h following H I [(52±8) cells/slice]. The expression of EAAT1 in the ipsilateral cortex to common carotid artery (CCA) ligation was higher than that in the contralateral cortex. CON￣CLU￣SION: After H I, the expression of EAAT1 had a temporal change in cerebral cortex of newborn rat, and was mainly located in the ipsilateral cortex to CCA ligation.

关 键 词：缺氧缺血性脑病 新生儿 脑内 谷氨酸载体 EAAT 

分 类 号：R722.12[医药卫生—儿科]