鉴别HCV C蛋白上HLA-A2限制的CTL识别表位的研究  

Identification of the eqitopes on HCV core protein recognized by HLA-A2-restricted cytotxic T lymphocytes

作　　者：周红超[1] 徐德忠[1] 张鹏[1] 闫永平[1] 张景霞[1] 赵小宁[1] 朱勇[2] 金伯泉[2] 

机构地区：[1]第四军医大学流行病学教研室,西安710032 [2]第四军医大学免疫学教研室,西安710032

出　　处：《细胞与分子免疫学杂志》1999年第1期10-13,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金!39570634

摘　　要：本文先用计算机预测、后用51Cr4h释放分析证实的方法，对丙型肝炎病毒（HCV）核心区蛋白（C蛋白）上，HLA－A2限制性CTL识别表位进行了鉴别。结果发现，2名感染HCV的HLA－A2阳性供血员的外周血单个核细胞（PBMc）对ALAHGVRAL（core150～158）肽段标记的自体靶细胞有溶解作用，杀伤率分别为37．5％和15．8％；用抗CD4mAb阻断后杀伤率无明显变化，而用抗CDSmAb阻断后明显减少，提示该条肽段可能为HLA－A2限制性CTL识别表位。In this Paper, we attempted to idenop epitopes recognized by the HLA-A2 metricted cytotobo T lymphocyte (CTL)on hepatihs C virus (HCV) core protein utilizing the methed of computer prwhction before conforming by 4 h 31Cr-release assay. The results showed that Peripheral bled mononuclear cells(PBMCS) Obtained fro two HLA-A2 posihve donors who infected with HCV could lyse autolognus target cells theied vitb peptide 'AraHGVRAL(core 150 ～ 158)'. The specific lysis of the cells from the two donors were 37. 5% and 15. 8%, mepechvely. Blocking of the CTL response with and-CD4 showed that the specific lysis had no signifcant decrease. But blocking of CTL resPOnse with and-CDS could abolish the lysis. h indicated that the peptide was the Candidate epitope recwind by the HLA-A2 restricted CTL.

关 键 词：丙型肝炎病毒 细胞毒性T细胞 TLA-A2 表位 

分 类 号：R373.2[医药卫生—病原生物学] R392.11[医药卫生—基础医学]