用RP-HPLC法测定黄芪毛状根中黄芪甲苷的含量  

Determination of Astragaloside Ⅳ in the Hairy Root Cultures of Membranous Milkvetch (Astragalus membranaceus) by RP-HPLC

作　　者：郑志仁[1] 宋纯清[1] 刘涤 胡之璧 

机构地区：[1]上海中医药大学中药学院中药生物工程研究室,200032

出　　处：《中草药》1999年第2期98-99,共2页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金;上海市科委和教委资助项目

摘　　要：ＲＰ－ＨＰＬＣ法对黄芪毛状根中黄芪甲苷进行定量分析，在实验条件下，黄芪甲苷标准品在 １０．６４～５３．２０μｇ范围内与峰面积呈良好的线性关系；平均加样回收率为９２．６６％；精密度试验相 对标准偏差为４．２０％和２．４０％；测得黄芪毛状根中黄芪甲苷的含量为０．１４％（干重）。An accurate HPLC method for the determination of astragaloside Ⅳ in the hairy root cultures of Astragalus membranaceus (Fisch. ) Bunge was developed. Calibration graphs was　rectilinear between 10. 64μg and 53.20μg under the experimental conditions. The recovery was 92.66% The relative standard deviations (RSDs) of measurement precision test were 4. 20% and 2. 4.0%. The astragaloside Ⅳ content measured in the haly root cultures was 0. 14% (dry weight).

关 键 词：HPLC 黄芪 毛状根 黄芪甲苷 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]