提高长春花冠瘿细胞中吲哚生物碱含量的研究  

Studies on the Enhancing Accumulation of Indole Alkaloids in Crown Gall Cells of Madagascar Periwinkle (Catharanthus roseus)

作　　者：王淑芳[1] 王宁宁[1] 王勇[1] 田俊英[1] 

机构地区：[1]南开大学生命科学学院,天津300071

出　　处：《中草药》1999年第2期130-132,共3页Chinese Traditional and Herbal Drugs

摘　　要：研究了长春花冠瘿细胞接种物年龄与接种量对悬浮培养细胞生物产量的影响，探讨了以大丽花轮枝孢菌的匀浆物为外源刺激物处理冠瘿细胞，对细胞生长和吲哚生物碱积累的作用。确立了外源刺激物产生效应的最适条件。Influences of cell age (at inoculation) and the amount inoculated on the biological mass production of cultured cells in Catharanthus roseus (L. ) G. Don suspension were investigated. Effects of treatment by Verticillium dahliae homogenate as elicitor on the cell growth and accumulation of indole alkaloids in the crown gall cells were studied and the optimal stimulation conditions were determined.

关 键 词：长春花冠瘿 外源刺激物 吲哚生物碱 细胞培养 

分 类 号：TQ464.9[化学工程—制药化工]