溴氰菊酯对大鼠脑细胞DNA的损伤作用  

DAMAGING EFFECT OF DELTAMETHRIN ON DNA OF RAT NEURAL CELLS

作　　者：牛玉杰[1] 石年[2] 严红[2] 刘毓谷[2] 

机构地区：[1]河北医科大学公共卫生学院劳动卫生学教研室 [2]同济医科大学环境毒理教研室

出　　处：《河北医科大学学报》1999年第2期65-68,共4页Journal of Hebei Medical University

基　　金：国家自然科学基金

摘　　要：目的研究溴氰菊酯（ＤＭ）对大鼠脑细胞ＤＮＡ的损伤作用。方法用流式细胞仪观察了ＤＭ处理后大鼠脑细胞ＤＮＡ片段化的发生情况；用荧光染色法观察了ＤＭ作用后大鼠分离脑细胞核的形态变化。结果用流式细胞仪进行检测，发现ＤＭ可以使大鼠脑组织皮层、海马和小脑部位ＤＮＡ片段化的细胞数明显增加；同时ＤＭ可以使分离的脑细胞ＤＮＡ受到损伤，细胞核出现染色质浓缩、边缘不整齐、碎裂或环状等类似凋亡的形态学改变。结论ＤＭ可以损伤脑细胞的ＤＮＡ，有可能诱发神经细胞的凋亡。Objective To study the damaging effects of deltamethrin(DM) on the DNA of rat neural cells.Methods With flow cytometry,the DNA fragmentation by DM in rat brain was measured.The morphological changes of nucleus by DM on rat dissociated neural cells were observed with fluorescent nuclear staining. Results Under the effect of DM, the number of DNA fragmental cells in cerebral cortex, hippocampus and cerebellum significantly increased,and the DNA of dissociated neural cells appeared some morphological changes like apoptosis. Conclusion DM may elicit apoptosis of rat neural cells through the DNA damage.

关 键 词：除虫菊酯 毒性 溴氰菊酯 脑细胞 DNA损伤 

分 类 号：TQ450.261[化学工程—农药化工] TQ456.3