肠道病毒RNA RT-PCR检测在病毒性心肌炎诊断中的应用  

USE OF RT PCR ASSAY IN DIAGNOSIS OF ENTEROVIRUS MYOCARDITIS

作　　者：王永祥[1] 金世香[1] 金玉怀[1] 刘贵霞[1] 

机构地区：[1]河北医科大学基础医学院微生物学与免疫学教研室

出　　处：《河北医科大学学报》1999年第2期79-80,共2页Journal of Hebei Medical University

摘　　要：目的探讨ＲＴ－ＰＣＲ方法在肠道病毒感染诊断中的应用。方法根据肠道病毒５’非编码区和ＶＰ２基因区共同序列设计一对引物，建立了检测各型肠道病毒的ＲＴ－ＰＣＲ方法。用该方法扩增已知病毒，并用于临床标本检测。结果所检测的１１种已知病毒中，只对肠道病毒的９个型别扩增。１８６例心肌炎患者血清标本中，５２例（２７．９５％）阳性。１５例脑炎患者脑脊液标本中，４例（２６．６７％）阳性。结论该ＲＴ－ＰＣＲ方法具有肠道病毒特异性，在肠道病毒感染的诊断中有一定应用价值。ObjectiveTo explore the use of RT PCR in diagnosis of enterovirus infection. MethodsA pair of premers hemologous to 5' non coding and VP 2 region of the enteroviruses was designed, and a RT PCR method of detecting all types of enteroviruses was developed. The serum samples of the patients with myocarditis and cerebrospinal fluid samples of the patients with meningitis were tested. ResultsAmong the 186 cases of myocarditis, 52 were positive for enterovirus RNA in serum. While 4 of 15 cases of meningitis were positive in cerebrospinal fluid.ConclusionDue to specificity on enterovirus,the RT PCR method has some value in the diagnosis of enterovirus infections.

关 键 词：心肌炎 诊断 病因学 肠道病毒感染 病毒性 PCR 

分 类 号：R542.210.4[医药卫生—心血管疾病]