镉对中华稻蝗保护酶系统的毒性效应  

Toxic Effects of Cd^(2+) on Protective Enzyme Systems in Different Developing Stage of Oxya chinensis(Orthoptera Acridoidae)

作　　者：李丽君[1] 郭亚平[2,3] 武文丽[1] 王跃[2] 马恩波[2] 

机构地区：[1]山西省农业科学院土壤肥料研究所,太原030006 [2]山西大学生物应用学研究所,太原030006 [3]山西大学生命科学学院,太原030006

出　　处：《农业环境科学学报》2010年第8期1448-1454,共7页Journal of Agro-Environment Science

基　　金：国家自然科学重大国际合作项目(30810103907);山西省农业科学院博士基金项目(YBSJJ0702)

摘　　要：采用急性染毒方法研究Cd2+胁迫对不同发育阶段中华稻蝗SOD、CAT、POD活性的影响。结果表明,在Cd2+的作用下,不同发育阶段中华稻蝗体内SOD、CAT、POD活性均发生变化。SOD、CAT、POD 3种酶对Cd2+有一定的耐受性:当Cd2+浓度在这3种酶的耐受范围内酶活性提高,反之酶活性降低。Cd2+对不同发育阶段虫体3种抗氧化酶的影响不同:3龄若虫SOD、POD活性最高;雌性成虫CAT活性最低,而3龄雄性若虫CAT活性最高。In order to clarify the toxic mechanism of Cd2＋ in Oxya chinensis,the toxic effect of Cd2＋ on the activities of three antioxidant en-zymes,superoxide dismutase（SOD）,catalase（CAT）and peroxidase（POD）,in different developing stage of O.chinensis injected with Cd were determined.Different developing stages O.chinensis were injected with Cd2＋ at different concentrations（0,50,100,150,200 mg.L-1）.The results showed that the effects of Cd2＋ on SOD,CAT and POD activities were different within the experimental gradient.Cd2＋ could induce rises of the activities of SOD,POD and CAT at some contents,and induce the decline of the activities at other contents.There were significant differences（P0.05）of SOD,CAT and POD activities among the treatments（except for SOD of males at the fifth-stage nymphs and adult O.chinensis）.The activities of CAT and POD probably depended on SOD activity to eliminate oxygen species（ROS）under Cd2＋ stress in the experiment.SOD and POD activities were the highest at the third instar nymphs,and the activity of CAT in female O.chinensis was the lowest at adults,and the activity of CAT in male O.chinensis was the highest at the third instar nymphs.These results indicated that the activities of SOD,CAT and POD in O.chinensis might act as indicative functions for Cd2＋ contamination and Cd2＋ could induce damage of antioxidant de-fense system in O.chinensis.

关 键 词：中华稻蝗 镉(CD) 超氧化物歧化酶(SOD) 过氧化氢酶(CAT) 过氧化物酶(POD) 

分 类 号：X503.223[环境科学与工程—环境工程]