人睫状神经营养因子的原核表达及其初步纯化  

Expression and preliminary purification of hCNTF gene

作　　者：江国春[1] 袁丽珍[1] 贾向旭[1] 张津辉[1] 

机构地区：[1]军事医学科学院放射医学研究所

出　　处：《军事医学科学院院刊》1999年第1期38-40,共3页Bulletin of the Academy of Military Medical Sciences

摘　　要：目的：优化条件实现人睫状神经营养因子（ｈｕｍａｎｃｉｌｉａｒｙｎｅｕｒｏｔｒｏｐｈｉｃｆａｃｔｏｒ，ｈＣＮＴＦ）ｃＤＮＡ在大肠杆菌中的高效表达。方法：ＣＮＴＦ克隆进ｐＢＶ２２０后，表达，凝胶过滤纯化，１０日龄鸡背根神经节（Ｅ１０ＤＲＧｓ）测定其生物活性。结果：ＣＮＴＦ在ＨＢ１０１中高效表达，并具有生物活性。结论：ＳＤＳ－ＰＡＧＥ观察到相对分子质量约为２４×１０３的预期表达产物，优化表达条件后发现其在ＨＢ１０１中的表达状况最好。Objective: To study high expression of the cDNA of human ciliary neurotrophic factor(hCNTF) in E.coli after obtaining the optimum expression conditions. Methods: CNTF cDNA was cloned into pBV220, and the expressed product was purified with gel filtration after it was highly expressed. Results: CNTF was highly expressed in HB101,which had biological activity after purification. Conclusion: The protein obtained was found to have a relative molecular weight of M r=24×10 3 by SDS PAGE observation and could promote growth of E10 DRG cells.

关 键 词：睫状神经 营养因子 纯化 表达 hCNTF 

分 类 号：Q78[生物学—分子生物学]