γ-干扰素对增生性瘢痕成纤维细胞影响的实验研究  

EFFECT OF γ INTERFERON ON THE FIBROBLASTS IN HYPERTROPHIC SCARS

作　　者：岑瑛[1] 严小蓉[1] 

机构地区：[1]华西医科大学附属第一医院整形烧伤科

出　　处：《中国修复重建外科杂志》1999年第2期122-126,共5页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的观察γ干扰素对增生性瘢痕及成纤维细胞的作用，探索γ干扰素治疗增生性瘢痕的依据。方法取１０例患者增生性瘢痕标本，培养增生性瘢痕的成纤维细胞，取第１代细胞，分为实验组和对照组。实验组加入γ干扰素１００Ｕ／ｍｌ，作用５天，并观察成纤维细胞的细胞计数、肌成纤维细胞分化比例及细胞凋亡率。结果实验组成纤维细胞计数、肌成纤维细胞分化比例下降，细胞凋亡率增加，与对照组比较有显著性差异（Ｐ＜０．０５）。结论γ干扰素抑制成纤维细胞生长，抑制向肌成纤维细胞分化，促进成纤维细胞凋亡。Objective To study the influence and mechanism of γ IFN on fibroblasts in hypertrophic scars(HTS). Methods The cultured fibroblastic cells were isolated from the hypertrophic scars of 10 patients. The fibroblasts were divided into two groups, one group was treated with γ IFN (100 U/ml, 5 days) and the other without γ IFN as control. The proliferative activity in both groups was investigated and compared by blood cytometer, the proportion of myofibroblast (MFB) and the ratio of apoptosis were examined and analysed between two groups by flow cytometry using α smooth muscle actin (α SMA) as marker.Results The proliferative activity was downregulated with γ IFN. In γ IFN treated group, the differentiation of MFB were reduced and the decreasing ratio was 3.2% at the 2nd day and up to 10.5% at the 8th day, then it reduced gradually. The apoptosic ratio is 17.7% in γ IFN treated group, and is 10.9% in control group. The difference was statistically significant.Conclusion γ IFN could downregulate the proliferation of fibroblasts, decrease the differentiation of MFB and induce the apoptosis. It has beneficial effect in the treatment of hypertrophic scars(HTS).

关 键 词：Γ干扰素 增生性瘢痕 成纤维细胞 细胞凋亡 

分 类 号：R619.6[医药卫生—外科学]