应用快速色谱技术构建少数民族大复方组分库的方法学研究  

Methodology of flash chromatography application in multi-component library construction of large ethnodrug compound prescription

作　　者：刘庆山[1] 张梓倩[1] 段云霞[1] 赵多明[1,2] 崔箭[1] 

机构地区：[1]中央民族大学中国少数民族传统医学研究院药物筛选室,北京100081 [2]兰州大学公共卫生学院卫生毒理学研究所,兰州730000

出　　处：《国际药学研究杂志》2010年第4期310-314,共5页Journal of International Pharmaceutical Research

基　　金：国家自然科学基金面上项目资助(30973959)

摘　　要：目的建立少数民族药大复方全组分的快速制备方法,为有效成分的高通量筛选提供大量样品。方法应用快速色谱仪,以蒙药白脉散乙酸乙酯提取部位为研究对象,采用线性梯度洗脱法快速划段制备一系列色谱组分,采用高效液相色谱法对分离效果进行评价。结果从该复方乙酸乙酯部位获得29个色谱组分,该方法对组方中不同性质的物质起到了较好的分离与富集效果。结论快速色谱技术可用于构建民族复方药物组分库,这将有利于攻克民族药大复方药效物质基础研究难题。Objective To establish the rapid method of multi-component library preparation of large ethnodrug compound prescription and offer large amounts of samples for active components high-throughput screening.Methods The ethyl acetate extract from Mongolian medicine BaiMai Powder was separated into a series of fractions by linear gradient elution with flash chromatography.The separation efficiency was evaluated by high-performance liquid chromatography.Results Twenty-nine sequential components were prepared from the ethyl acetate extract of the prescription,enrichment and separation of compounds with different nature were well complished.Conclusion Multi-component library of ethnodrug compound prescription can be constructed by flash chromatography.This method will help to overcome the difficulty of the pharmacodynamic chemical substances research of large ethnodrug compound prescription.

关 键 词：快速色谱 组分库 民族药 复方 高通量筛选 

分 类 号：R284[医药卫生—中药学]