基于硫堇电聚合膜的甲胎蛋白免疫传感器  

An Elcctrochemical α-Fetoprotein Immunosensor Based on Electropolymerized Thionine Film

作　　者：姜丽[1,2] 徐华筠[1,2] 赵常志[1,2] 焦奎[1,2] 

机构地区：[1]青岛科技大学生态化工教育部重点实验室 [2]化学与分子工程学院,山东青岛266042

出　　处：《青岛科技大学学报（自然科学版）》2010年第4期331-335,共5页Journal of Qingdao University of Science and Technology:Natural Science Edition

基　　金：国家自然科学基金资助项目(20635020)

摘　　要：将硫堇电聚合到铂电极表面形成聚硫堇膜,通过戊二醛自组装蛋白质,在酶标抗体与相应的抗原发生免疫反应时催化底物氧化聚硫堇。根据聚硫堇膜电极的还原电流表达抗原的量,制得高灵敏度的甲胎蛋白免疫传感器。通过循环伏安法及差示脉冲伏安法考察了电极表面的电化学行为,并对免疫传感器的性能进行了详细研究。在优化的实验条件下,伏安法检测甲胎蛋白的线性范围为5.00～200 ng.mL-1,线性相关系数0.998 7,检测限3.70 ng.mL-1。An elcctrochemical α-fetoprotein immunosensor was constructed by using an electropolymerized thionine film on a platinum electrode.The amino groups of thionine were used to covalently attach protein probes via the aid of glutaraldehyde cross-linking,leading to significantly improved detection stability compared with conventional entrapment methods.Under the optimized conditions,the immunosensor showed a specific response to α-fetoprotein in the range of 5.00 to 200 ng·mL^-1 with a detection limit of 3.70 ng·mL^-1,and the linearly dependent coefficient was 0.998 7.

关 键 词：免疫传感器 甲胎蛋白 聚硫堇 

分 类 号：O657.1[理学—分析化学]