天麻AFLP优化体系中的引物筛选  

Primers screening in gastrodia elata AFLP optimizing system

作　　者：张弦 张小蕾[2] 谢渊[3] 李毅[3] 

机构地区：[1]贵州电力医院检验科,贵州贵阳550002 [2]贵阳医学院检验系,贵州贵阳550004 [3]贵阳医学院分子生物实验室,贵州贵阳550004

出　　处：《中国当代医药》2010年第24期6-8,共3页China Modern Medicine

基　　金：贵州省中药现代化科技产业研究开发专项项目;合同编号:黔科合中药专字[2003]22号;项目名称:天麻及共生菌蜜环菌的DNA指纹图谱研究;贵州省贵阳市科学技术计划项目;合同书:[2004]筑科农字第21号;项目名称:天麻及共生菌蜜环菌的DNA指纹图谱研究

摘　　要：目的:筛选引物组合,建立天麻DNA指纹图谱研究中能对反应产物进行分组分析且稳定、可靠的天麻AFLP技术平台。方法:依托天麻AFLP优化体系,筛选出选择性引物组合,进而确定预扩增引物组合、接头及限制性内切酶组合。结果:将筛选出的引物组合应用到天麻AFLP反应优化体系,得到的产物能在1.5%琼脂糖凝胶电泳图谱上生成清晰、完整、可分离的谱带,多态性好且便于分析。结论:筛选出的引物组合利于建立能对反应产物进行分组分析的、标准化的天麻AFLP反应程序。Objective：To screen primer combination for establishing a stable and reliable gastrodia AFLP technology platform which can carry on division analysis of reaction products in gastrodia DNA fingerprints mapping research.Methods：To screen out selective Primer combination and ensure pre-amplification,adapter connector as well as restrictive incision enzyme combination.Results：The reaction products derived from AFLP reaction system to which selected combination is applied can produce clear,complete and detachable band on the 1.5% agarose gel electrophoresis.And this band is polymorphic and easy to be analyzed.Conclusion：The selected combination is beneficial to establish an easy analysis and standard gastrodia AFLP reaction system for the reaction products.

关 键 词：天麻 AFLP 引物筛选 

分 类 号：R282.710.3[医药卫生—中药学]