耐三唑类杀菌剂木霉突变体TUV-13遗传稳定性的研究  

Genetic Stability of Trichoderma harzianum Mutant TUV-13 with Resistance to Triazole Fungicide

作　　者：安哲宇 梁志怀 魏林[1,2] 罗秀娟[1] 陈玉荣 

机构地区：[1]中南大学研究生院隆平分院,湖南长沙410125 [2]湖南省植物保护研究所,湖南长沙410125

出　　处：《湖南农业科学》2010年第3期1-3,6,共4页Hunan Agricultural Sciences

基　　金：湖南省博士后科研资助专项计划项目(2008RS4028);湖南省农村科技计划项目(07NK4121);国家"十一五"科技支撑计划项目(2006BAD17B08)

摘　　要：应用紫外诱变和含药培养基诱导相结合的方法,获得了一株对三唑类杀菌剂有较好耐药性的哈茨木霉突变体TUV-13。分别测定该突变体继代培养的5代,10代和20代对腈菌唑耐受能力和对病原菌的拮抗性,结果表明:该突变体的抗药性可以稳定遗传,且对病原菌的拮抗性也未丧失。同时,应用RAPD分子技术分析了不同世代菌株间的相似性。Using combined method of UV-induced mutage nesis and fungicide-containing medium to obtain a Trichoderma harzianum mutant TUV-13 with strong resistance to triazole fungicide.The resistant ability and the antagonistic ability of subcultural 5th generation,10th generation and 20th generation of TUV-13 to myclobutanil and pathogen were determined respectively.The results showed that the fungicide resistance of TUV-13 could be stably inherited,and the antagonistic ability to pathogen had not lost.Then,the similarity among different generation strains was analyzed by RAPD molecular technology.

关 键 词：木霉 腈菌唑 耐药性 RAPD 水稻病害 

分 类 号：Q949.32[生物学—植物学]