Influence of L-Cysteine Concentration on Oxidation-reduction Potential and Biohydrogen Production  

作　　者：陈火晴 马晓轩 范代娣 骆艳娥 高鹏飞 杨婵媛 

机构地区：[1]Shaanxi Key Laboratory of Degradable Biomedical Materials,School of Chemical Engineering,Northwest University,Xi'an 710069,China [2]Shaanxi R&D Center of Biomaterials and Fermentation Engineering,School of Chemical and Engineering,Northwest University,Xi'an 710069,China

出　　处：《Chinese Journal of Chemical Engineering》2010年第4期681-686,共6页中国化学工程学报（英文版）

基　　金：Supported by the National High Technology Research and Development Program of China（2006AA02Z246 2007AA03Z456）; the National Natural Science Foundation of China（20776119）; the Specialized Research Fund for the Doctoral Program of Higher Education of China（20096101120023）; Shaanxi Provincial Natural Science Foundation（SJ08B03）; Shaanxi Key Subject Program China

摘　　要：The effects of L-cysteine concentration on biohydrogen production by Enterobacterium Bacterium M580 were investigated in batch cultivation.The experimental results showed that L-cysteine could enhance the cell growth,hydrogen production rate and hydrogen yield when its concentration was less than 500 mg·L-1,while it had negative effects when its concentration was higher than 500 mg·L-1.The hydrogen production was the highest 1.29 mol·mol-1(H2/glucose) when 300 mg·L-1L-cysteine was added into the culture,and the yield was 9.4% higher than that in the control.The oxidation-reduction potential(ORP) ,which was influenced by L-cysteine,also affected hydrogen production.The ORP values were in the range-300 mV to-150 mV when the L-cysteine concentration was higher than 500 mg·L-1.Although the ORP in this range was favorable for hydrogen production,it was not suitable for the biomass growth.Hence,less hydrogen was produced.When the L-cysteine concentration was lower than 500 mg·L-1,the ORP was more suitable for both biomass growth and hydrogen production.In addition,at least 91%glucose was consumed when L-cysteine was added to the culture media,compared to the 97.37% consumption without L-cysteine added.The effects of L-cysteine concentration on biohydrogen production by Enterobacterium Bacterium M580 were investigated in batch cultivation.The experimental results showed that L-cysteine could enhance the cell growth,hydrogen production rate and hydrogen yield when its concentration was less than 500 mg·L^-1,while it had negative effects when its concentration was higher than 500 mg·L^-1.The hydrogen production was the highest 1.29 mol·mol^-1（H2/glucose） when 300 mg·L^-1L-cysteine was added into the culture,and the yield was 9.4% higher than that in the control.The oxidation-reduction potential（ORP） ,which was influenced by L-cysteine,also affected hydrogen production.The ORP values were in the range-300 mV to-150 mV when the L-cysteine concentration was higher than 500 mg·L^-1.Although the ORP in this range was favorable for hydrogen production,it was not suitable for the biomass growth.Hence,less hydrogen was produced.When the L-cysteine concentration was lower than 500 mg·L^-1,the ORP was more suitable for both biomass growth and hydrogen production.In addition,at least 91%glucose was consumed when L-cysteine was added to the culture media,compared to the 97.37% consumption without L-cysteine added.

关 键 词：anaerobic fermentation BIOHYDROGEN L-CYSTEINE ORP 

分 类 号：TQ116.2[化学工程—无机化工] X703[环境科学与工程—环境工程]