急性肺损伤大鼠肺表面活性物质相关蛋白A mRNA表达与支气管肺泡灌洗液肿瘤坏死因子-α、白细胞介素-8水平的相关性  

Correlation between Expression of Pulmonary Surfactant Associated Protein A mRNA in Lung Tissue and the Levels of Tumor Necrosis Factor-α and Interleukin-8 in Bronchoalveolar Lavage Fluid in Rats with Acute Lung Injury

作　　者：李红日[1] 宋国维[1] 崔小岱[2] 孙春荣[2] 伏瑾[2] 孙丽萍[2] 

机构地区：[1]首都儿科研究所附属儿童医院急诊科,北京100020 [2]首都儿科研究所临床中心实验室,北京100020

出　　处：《实用儿科临床杂志》2010年第16期1216-1218,共3页Journal of Applied Clinical Pediatrics

基　　金：国家科技攻关计划课题(2003BA712A11-10)

摘　　要：目的探讨急性肺损伤(ALI)大鼠肺表面活性物质相关蛋白A(SP-A)mRNA表达与支气管肺泡灌洗液(BALF)中肿瘤坏死因子-α(TNF-α)、白细胞介素-8(IL-8)水平的相关性。方法大肠埃希杆菌[O111B4,(4.4～5.6)×1012CFU·L-1]3mL·kg-1气管注射制作大鼠肺内源性ALI模型(模型组),对照组气管注射3mL·kg-19g·L-1盐水。气管注射大肠埃希杆菌后24h行动脉血气分析,ELISA法检测BALF中TNF-α、IL-8水平,反转录-PCR法检测肺组织SP-A mRNA表达,并与TNF-α、IL-8行相关性分析。结果 1.模型组动脉血氧分压[pa(O2):(7.71±1.27)kPa]明显低于对照组[(13.03±0.92)kPa](P=0.000)。2.模型组BALF中TNF-α、IL-8水平[(409.03±82.77)ng·L-1,(80.76±25.03)ng·L-1]均明显高于对照组[(108.92±17.35)ng·L-1,(32.54±12.10)ng·L-1](Pa=0.000)。3.肺组织SP-AmRNA表达(0.31±0.15)明显低于对照组(0.66±0.16)(P=0.000)。4.SP-A mRNA表达与TNF-α水平呈显著负相关(r=-0.626,P=0.01),与IL-8水平无显著相关性(r=-0.207,P=0.441)。结论炎性因子TNF-α、IL-8在ALI发病机制中起重要作用,ALI肺组织SP-A mRNA表达明显下降,且与BALF中TNF-α水平呈显著负相关。Objective To explore the correlation between expression of pulmonary surfactant associated protein A（SP-A） mRNA in lung tissue and the levels of tumor necrosis factor alpha（TNF-α） and interleukin-8（IL-8） in bronchoalveolar lavage fluid（BALF） in rats with acute lung injury（ALI）.Methods ALI model was induced by intratracheal escherichia coli injection[3 mL·kg-1,O111B4,（4.4-5.6）×1012CFU·L^-1）],saline was administered intratracheally in the control group.Arterial oxygen partial pressure[pa（O2）] was recorded 24 h after escherichia coli and saline injection,TNF-α and IL-8 level in BALF were measured by enzyme-linked immunosorbent assay,lungs were fixed with formalin to perform pathological examination.The expression of SP-A mRNA in lung tissue was detected by reverse transcription-polymerase chain reaction.The correlation between the expression of SP-A mRNA and the levels of TNF-α and IL-8 were analyzed.Results 1.The pa（O2） in model group was significantly lower than that in control group[（7.71±1.27） kPa vs （13.03±0.92） kPa,respectively] （P=0.000）.2.The levels of TNF-α and IL-8 in model group were（409.03±82.77） ng·L^-1 and （80.76±25.03） ng·L^-1,respectively,which were significantly higher than those in control group [（108.92±17.35） ng·L^-1 and （32.54±12.10） ng·L^-1,respectively ]（Pa=0.000）.3.The expression of SP-A mRNA in model group was significantly lower than that in control group [（0.31±0.15） vs （0.66±0.16）] （P=0.000）.4.There was a negative correlation between the expression of SP-A mRNA in lung tissue and the level of TNF-α in the BALF（r=-0.626,P=0.01）,but no correlation was found between the expression of SP-A mRNA in lung tissue and IL-8 in the BALF（r=-0.207,P=0.441）.Conclusions TNF-α and IL-8 play important roles in ALI.The expression of SP-A mRNA in lung tissue was decreased significantly,and there was a negative correlation between the expression of SP-A mRNA in lung tissue and the level of TNF-α in the

关 键 词：急性肺损伤 肺表面活性物质相关蛋白A 细胞因子 大肠埃希杆菌 

分 类 号：R725.6[医药卫生—儿科]