离体培养条件对卷叶贝母鳞茎再生的影响  

The Effects of in vitro Culture Conditions on Regeneration of Fritillaria cirrhosa

作　　者：王跃华[1] 代勇 何宗晟[1] 孙雁霞[1] 闫胜杰[1] 徐世军 王晓蓉 

机构地区：[1]成都大学生物产业学院,四川成都610016 [2]成都恩威集团(投资)有限公司,四川成都610041

出　　处：《中药材》2010年第6期854-856,共3页Journal of Chinese Medicinal Materials

基　　金：国家"十一五"科技支撑计划重点项目(2006BAI06A13);四川省科技支撑计划(2010SZ0007)

摘　　要：目的:筛选出卷叶贝母鳞茎再生的最佳离体培养条件。方法:通过对卷叶贝母开花期鳞茎的离体培养,研究了不同光照条件、培养温度、激素配比以及病毒唑用量对卷叶贝母鳞茎再生的影响。结果与结论:卷叶贝母鳞茎外植体进行适当的低温暗处理后,再转入光下培养,可明显地减轻外植体的褐化程度;鳞茎再生的最适培养温度为(20±2)℃,激素对鳞茎再生有明显的促进作用,其中以6-BA 2.0 mg/L和NAA 0.2 mg/L组合为最佳;浓度为10 mg/L的病毒唑最适于鳞茎的再生。Objective： The optimal in vitro culture conditions for the regeneration of Fritillaria cirrhosa were screened.Methods： Through the in vitro culture of the flowering stage Fritillaria cirrhosa,the effect of illumination,culture temperature,hormone combination and the amount of ribavirin on the regeneration of Fritillaria cirrhosa were studied.Results and Conclusion： The explant browning level could be apparently reduced if the bulb were cultured under the ray after exposure to proper low-temperature.The optimal temperature for the regeneration of bulb is（20 ± 2） ℃.The hormone has obvious promotional effect on the regeneration of bulb,and the best combination is 6-BA 2.0 mg/L plus NAA 0.2 mg/L.Concentration of 10 mg/L of ribavirin is best for the regeneration of bulb.

关 键 词：离体培养条件 卷叶贝母 鳞茎再生 

分 类 号：R282.2[医药卫生—中药学]