地鳖纤溶活性蛋白的溶栓、溶血与抑瘤作用研究  

The Research of Thrombolytic and Hemolysis Effect from Eupolyphage Fibrinolyric Protein and Its Inhibitory Effect on S_(180) Ascites Tumor of Mice

作　　者：黎子蔚[1] 韩雅莉[1] 刘浩[1] 丁鸿[1,2] 

机构地区：[1]广东工业大学轻工化工学院,广东广州510006 [2]揭阳职业技术学院,广东揭阳522000

出　　处：《中药材》2010年第6期859-863,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金项目(C30772739);广东工业大学轻工化工学院"211工程"培育项目

摘　　要：目的:从地鳖虫中分离纯化得到纤溶活性蛋白(EFP),并研究其溶栓、溶血及对小鼠S180腹水瘤细胞的抑制作用。方法:通过硫酸铵分段盐析、DEAE-纤维素柱层析等方法从地鳖虫中分离纯化得到一种地鳖虫纤溶活性蛋白,对其进行了溶栓、溶血实验,采用MTT法和集落形成法研究了EFP对小鼠S180腹水瘤细胞的抑制作用。结果:分离得到的EFP分子量为87.2 kDa,具有一定的溶栓和溶血作用,并且其溶栓和溶血作用都具有浓度依赖性,低浓度(≤0.12mg/mL)的EFP不具有溶血作用。且EFP对S180腹水瘤细胞生长及集落形成都有抑制作用。结论:地鳖纤溶活性蛋白具有较强溶栓活性和微弱的溶血性,且对小鼠S180腹水瘤细胞有一定的抑制作用。Objective： Eupolyphage fibrinolyric protein（EFP） was isolated and purified from Eupolyphage sineses,and its thrombolytic effect,hemolysis effect and inhibitory effect on S180 ascites tumor were investigated.Methods： EFP was isolated and purified by ammonium precipitation and DEAE ion exchange chromatography.It′s thrombolytic and hemolysis effect were determined.MTT method and Colony-forming method were used to determine the inhibitory effect on S180 ascites tumor.Results： the EFP was proved to have the effect of Thrombolytic and Hemolysis,and both increased dose-depengdently,however at a lower concentration,the EFP had no hemocytolysis.The EFP was also proved the effect of inhibitory on cell proliferation and Colony-forming on S180 ascites tumor of Mice.Conclusion： EFP has a strong thrombolytic activity and weak hemolytic,and has inhibitory effect on S180 ascites tumor of mice.

关 键 词：地鳖 纤溶活性 溶血栓 溶血 小鼠S180腹水瘤 抑瘤作用 

分 类 号：R282.74[医药卫生—中药学]