天冰调督胶囊对慢性点燃癫痫大鼠学习记忆行为及海马NMDAR1表达的影响  

Effects of Tianbingtiaodu Capsule on Improvement of Learning and Memory Abilities and Expression of NMDAR1 of Epileptic Rats in the Hippocampus

作　　者：王渝[1] 裴林[2] 刘春燕[2] 裴逸然[3] 贾晓明[2] 

机构地区：[1]北京大学深圳医院,广东深圳518036 [2]河北省中医药研究院,河北石家庄050031 [3]南开大学生命科学学院,天津300071

出　　处：《中药材》2010年第6期947-951,共5页Journal of Chinese Medicinal Materials

基　　金：国家中医药管理局重点研究室资助项目(ZD200823-10);河北省科技攻关计划项目(99276217D)

摘　　要：目的:观察天冰调督胶囊对印防己毒化学点燃癫痫模型大鼠学习记忆行为学改变及海马组织NMDAR1表达的影响。方法:SD大鼠腹腔注射印防己毒素(1.5 mg/kg)连续30 d,制作反复发作癫痫模型,点燃模型大鼠随机分为模型组、天冰调督胶囊低(0.4 g/kg)、高(0.8 g/kg)剂量组、丙戊酸钠组及拉莫三嗪组,采用Morris水迷宫检测各组大鼠学习记忆行为学,RT-PCR检测海马组织NMDAR1 mRNA,Western-blot和免疫组化法检测NMDAR1蛋白表达情况。结果:治疗60 d后,与模型组比较,天冰调督胶囊低、高剂量组大鼠水迷宫实验寻找平台时间显著缩短(P<0.05或P<0.01),通过平台次数显著增加(P<0.01);NMDAR1 mRNA含量均增加(P<0.05或P<0.01);在CA3、DG区NMDAR1表达均增强(P<0.05)。结论:天冰调督胶囊可能通过调节NMDAR1水平改善癫痫学习记忆等认知障碍。Objective：To study effects of Tianbingtiaodu capsule on changement of learning and memory abilities and expression of NMDAR1 in epileptic rats.Methods：Picrotoxin（1.5 mg/kg） was injected intraperitonrally in SD rats for thirty days.After the repeated attacks epilepsy model had been established successfully,model rats were randomly divided into model group,Tianbingtiaodu capsule low dosage（0.4 g/kg） group,Tianbingtiaodu capsules high dosage（0.8 g/kg） group,Piracetam group and Piracetam and Valproate group.All groups were fed everyday.Sixty days later,the learning and memory abilities were tested with Morris water maze method.Expression of NMDAR1 in hippocampus was observed with western-blot and immunohistochemical method.The expression of NMDAR1 mRNA in hippocampus was determined by RT-PCR.Results：Compared to the control group,the learning and memory abilities were significantly lower（P0.01）,immune positive remarks of expression of NMDAR1 in hippocampus AC3 significantly increased in model group（P0.05）;Compared with model group,the learning and memory abilities and expression of NMDAR1 in hippocampus was improved sinificantly in Tianbingtiaodu group（P0.05 or P0.01）.Conclusion：Tianbingtiaodu capsule could improve the impairment of learning and memory in rats after repeated attacks of epilepsy through adjusting the NMDAR1 expression.

关 键 词：天冰调督胶囊 癫痫大鼠 学习记忆 NMDAR1 

分 类 号：R285.5[医药卫生—中药学]