VEGF及PCNA在稽留流产组织中的表达及临床意义  

Expression of VEGF and PCNA in Tissues from Missed Abortion Patients and Its Clinical Significance

作　　者：任永变[1] 薛瑞娟[1] 

机构地区：[1]延安大学附属医院产科,陕西延安716000

出　　处：《现代检验医学杂志》2010年第4期55-57,共3页Journal of Modern Laboratory Medicine

摘　　要：目的 研究血管内皮生长因子（VEGF）及增殖性细胞核抗原（PCNA）在稽留流产绒毛组织中的表达及其临床意义,探讨VEGF和PCNA在稽留流产发生发展中的作用.方法 免疫组化SP法检测57例稽留流产组织中VEGF,PCNA蛋白表达水平;以GAPDH作为内参,实时定量PCR法检测稽留流产组织中其mRNA的表达水平,并与53例正常流产组织作对比.结果 VEGF及PCNA在正常流产组织中的蛋白表达水平明显高于稽留流产患者,差异有统计学意义（P＜0.01）;两者mRNA的表达水平在正常流产组织中也显著高于实验组（P＜0.01）,与蛋白表达水平有一致性.结论 VEGF及PCNA在稽留流产组织中表达水平明显降低,与正常流产组织相比有明显差异,结果提示VEGF及PCNA可能与稽留流产的发生有关,有可能作为判断稽留流产的参考指标,值得进一步研究.Objective To study the expression and clinical significance of VEGF and PCNA in missed abortion and explore their effects on missed abortion. Methods 57 cases of missed abortion cases were detected for the expression of VEGF and PCNA by means of immunohistochemical SP;GAPDH as inner reference,measuring the mRNA expression of VEGF and PCNA by real-time PCR,compared to normal abortion. Results The expression and mRNA level of VEGF and PCNA from normal abortion were more than that of missed abortion,coherent to that of protein expression. Conclusion VEGF and PCNA may play a role in progression of missed abortion,and may be used as a useful marker to judging missed abortion. It is worth to study further.

关 键 词：稽留流产 血管内皮生长因子 增殖性细胞核抗原 

分 类 号：R714.21[医药卫生—妇产科学] R392.11[医药卫生—临床医学]