人参皂甙Rg1对NOS的调控在海马神经元放射性损伤防护中的意义  

Ginsenoside Rg1 protects rat hippocampal neurons from radiation injury by regulating NOS activity

作　　者：陈勇[1] 孙爱民[1] 陈智贤[1] 刘英[1] 陈龙华[1] 袁亚维[1] 

机构地区：[1]南方医科大学南方医院放疗科,广东广州510515

出　　处：《南方医科大学学报》2010年第7期1522-1525,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30670633;30973846)

摘　　要：目的研究人参皂甙Rg1对原代培养大鼠海马神经元放射性损伤的保护作用及机制,为放射性脑损伤的预防提供理论依据及新的方法。方法 30Gy的X射线单次照射培养至12d的海马神经元,用DAPI染核方法检测海马神经元凋亡情况,用NOS测定试剂盒测定细胞培养液NOS活性。结果 30Gy组在照射后24h核固缩百分数为(25.3±3.57)%,较0Gy组(1.95%±0.78%)有显著性差异(P<0.01);30Gy+人参皂甙Rg120mol/L组在照射后24h核固缩百分数为(7.43±1.51)%,较30Gy组(P<0.01)及0Gy组(P<0.01)均有显著性差异。30Gy组在照射后24h细胞培养液NOS活性为(6.46±0.95)U/ml,较0Gy组[(3.20±0.70)U/ml]有显著性差异(P<0.01),30Gy+人参皂甙Rg120mol/L组在照射后24h NOS活性为(3.85±0.69)U/ml,较30Gy组(P<0.01)及0Gy组(P<0.05)均有显著性差异。结论应用人参皂甙可以通过降低X线照射后NOS活性而显著减少神经元的凋亡。Objective To study the protective effect of ginsenoside Rg1 on cultured rat hippocampal neurons against radiation injury and explore new therapies for preventing radiation encephalopathy. Methods Rat hippocampal neurons cultured for 12 days were subjected to a single-dose X-ray exposure of 30 Gy. 4＇,6-diamidino-2-phenylindole （DAPI） staining was used to detect the neuronal apoptosis and NOS activity kit utilized to evaluate NOS activity in the cells after the exposure. Results Nuclear condensation was detected in （25.3±3.57）% of the neurons 24 h after 30 Gy X-ray exposure, a rate significantly higher than that in the control cells [（1.95±0.78）%, P0.01]. In the neurons pretreated with ginsenoside Rg1, only （7.43±1.51）% of the cells presented with nuclear condensation after the exposure, significantly different from the rates in the control cultures and the exposed cultures （P0.01）. The NOS activity of exposed cultures was 6.46±0.95 U/ml, significantly higher than that in the control cultures （3.20±0.70 U/ml, P0.01）. The NOS activity of the neurons pretreated with ginsenoside Rg1 was 3.85± 0.69 U/ml , significantly different from that in the control cultures （P0.05） and the exposed cultures （P0.01）. Conclusion Ginsenoside Rg1 offers significant protective effect on rat hippocampal neurons from radiation-induced apoptosis by reducing the activity of NOS.

关 键 词：人参皂甙RG1 一氧化氮合酶 海马神经元 放射性脑损伤 

分 类 号：R818[医药卫生—放射医学]