慢性乙型肝炎患者外周血CD8^+T淋巴细胞部分免疫效应分子的表达特征  

Expression characteristics of some immune effector molecules in CD8～+ T lymphocytes from patients with chronic hepatitis B

作　　者：高月求[1] 姚嫣[1] 李曼[1] 

机构地区：[1]上海中医药大学附属曙光医院肝肾疾病病证教育部重点实验室,上海200021

出　　处：《南方医科大学学报》2010年第7期1606-1609,共4页Journal of Southern Medical University

基　　金：上海市科委引导项目(074119608);上海市教委E研究院计划项目(03009-8);上海市浦东新区创新项目(PKJ2007-Y23);上海市教育委员会重点学科(第五期)建设资助项目(J50307)

摘　　要：目的研究慢性乙型肝炎(CHB)患者外周血CD8+T淋巴细胞穿孔素(PF)、颗粒酶B(GrB)、颗粒溶素(GNLY)、肿瘤坏死因子-α(TNF-α)和γ-干扰素(IFN-γ)的表达特征及其与感染状态的相关性。方法通过检测ALT、AST和TB及HBVDNA拷贝数评估CHB患者的感染状态。健康志愿者作为对照组,CHB患者根据感染状态分为肝功能正常高病毒复制组、肝功能正常低病毒复制组、肝功能异常高病毒复制组、肝功能异常低病毒复制。应用流式细胞法检测外周血CD8+T细胞免疫效应分子的表达水平,分析其与感染状态的相关性。结果肝功能正常低病毒复制组CD8+T细胞表达的GrB、TNF-α、IFN-γ明显高于肝功能异常高病毒复制组(P<0.05);肝功能正常高病毒复制组的IFN-γ高于肝功能异常高病毒复制组(P<0.05);PF、GNLY在各组中的表达无明显差异(P>0.05);GrB、PF、GNLY、TNF-α、IFN-γ两两呈正相关。结论 CHB患者CD8+T淋巴细胞表达的GrB、TNF-α和IFN-γ与病毒承载量、肝损伤呈负相关。Objective To investigate the expressions of perforin （PF）, granzyme B （GrB）, granulysin （GNLY）, TNF-α and IFN-γ in peripheral CD8＋ T lymphocytes and their correlation to infection status in patients with chronic hepatitis B （CHB）. Methods ALT, AST, TB and HBV DNA copy were detected to evaluate the infection status in CHB patients, with healthy volunteers serving as the control group. According to the infection status, the CHB patients were divided into 4 groups, namely normal hepatic function and high HBV DNA level group, normal hepatic function and low HBV DNA level group, abnormal hepatic function and high HBV DNA level group and abnormal hepatic function and low HBV DNA level group. The expressions of some immune effector molecules in CD8＋T cells were detected by flow cytometry, and the correlations between these immune effector molecules and the infection status were analyzed. Results The expressions of GrB, TNF-α and IFN-γ in normal hepatic function and low HBV DNA level group were significantly higher than those in abnormal hepatic function and high HBV DNA level group （P0.05）. The expression of IFN-γ in normal hepatic function and high HBV DNA level group was significantly higher than that in abnormal hepatic function and high HBV DNA level group （P0.05）. The expressions of PF and GNLY were similar among all the 4 groups. Positive correlations were noted between GrB, PF, GNLY, TNF-α and IFN-γ. Conclusion GrB, TNF-α and IFN-γ in peripheral CD8＋ T cells are inversely correlated to hepatic dysfunction and HBV DNA level in CHB patients.

关 键 词：慢性乙型肝炎 T淋巴细胞 穿孔素 颗粒酶B 颗粒溶素 肿瘤坏死因子-α Γ-干扰素 

分 类 号：R512.62[医药卫生—内科学]