高繁殖力黄淮山羊大卵泡颗粒细胞上调表达基因的研究  

Study on Different Gene Expression in Follicular Granulose Cells of Huanghuai Goats with High Prolificacy

作　　者：庞训胜[1,2] 王子玉[1] 应诗家[1] 张艳丽[1] 吴勇聪[1] 闫益波[1] 孟立[1] 钟部帅[1] 王锋[1] 

机构地区：[1]南京农业大学羊业科学研究所,南京210095 [2]安徽科技学院,凤阳233100

出　　处：《畜牧兽医学报》2010年第8期955-961,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技支撑计划课题(2008BADB2B04;2008BADB2B09);江苏省高技术研究项目(BG2007324)

摘　　要：旨在对山羊卵巢有腔卵泡发育中基因表达特点进行研究。本研究应用抑制消减杂交技术(SSH)对黄淮山羊卵泡期卵巢非闭锁大卵泡(6 mm)和小卵泡(4 mm)颗粒细胞内差异表达基因进行了研究,建立了消减cDNA文库,通过斑点杂交分析,从正向文库中随机挑取96个克隆进行差异表达的筛选,结果发现,其中有8个与已知功能基因高度相似,12个全新的表达序列标签(EST)。结果显示,这些基因可能影响着山羊卵泡的发育成熟和排卵。The aim of this study was to analyze the gene expression during the goat antral follicle development.The different expressed genes in granulosa cells between non-latching large follicles（LF,6 mm） and small follicles（SF,4 mm） during follicular phase of Huanghuai goat ovary were studied using suppressive subtractive hybridization（SSH）,and reduction cDNA library were also established.Furthermore,96 clones randomly picked from the forward library were screened for differential expression by dot blot analysis.The results showed that eight clones had highly similar with the known genes,and twelve clones were new expressed sequence tags（ESTs）.The results indicated that these genes may influence the maturation and ovulation of goat follicle.

关 键 词：黄淮山羊 卵泡颗粒细胞 抑制消减杂交 基因表达 

分 类 号：S827[农业科学—畜牧学] Q344.5[农业科学—畜牧兽医]