Vero细胞共培养体系对猪胚胎早期发育的影响  

Effect of Co-culture System with Vero Cells on the Development of Porcine Embryos

作　　者：付博[1] 马红[1,2] 任亮[1] 赵金凤[1] 房庆昌[1] 郭镇华[2] 李忠秋[2] 刘娣 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030 [2]黑龙江省农业科学院,哈尔滨150086

出　　处：《畜牧兽医学报》2010年第8期962-966,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金："十一五"国家科技支撑计划重点项目(2008BADB2B02);转基因生物新品种培育重大专项(2008ZX08006-003)

摘　　要：本研究旨在探讨Vero细胞共培养体系对猪胚胎早期发育的影响。收集屠宰场废弃卵巢,抽取卵母细胞。采用电激活联合化学激活的方法得到孤雌胚胎,同时用所得的卵母细胞与卵丘细胞构建猪体细胞核移植重构胚;并将所得的孤雌胚、核移植重构胚移入NCSU-23(培养液)中与Vero细胞共培养。结果,Vero细胞共培养组的囊胚率显著高于对照组(P<0.05),各组囊胚细胞数无显著差异(P>0.05)。结果表明,Vero细胞作为共培养体系中的滋养层细胞有利于猪胚胎的早期发育。This study was conducted to investigate the effect of co-culture system with Vero cells on the development of porcine embryos.Ovaries were recovered from slaughterhouse,and oocytes were extracted.Oocytes were activated with electrical stimulation combined with chemical stimulation,then parthenogenetic embryos were obtained.Cloned embryos were constructed with porcine cumulus cells and oocytes.Cloned embryos and parthenogenetic embryos were co-cultured with Vero cells in NCSU-23（culture medium）.The results showed that groups co-cultrued with Vero cells achieved significantly higher blastocyst formation rate compared with control（P〈0.05）,while there was no significant difference for the cell number of blastocysts（P〉0.05）.The result indicated that co-culture system with Vero cells improved the development of porcine embryos in vitro.

关 键 词：猪 VERO细胞 共培养体系 孤雌胚 体细胞核移植 

分 类 号：S814.8[农业科学—畜牧学] Q813.1[农业科学—畜牧兽医]