10株鸭源新城疫病毒的分离鉴定及F基因遗传进化分析  

Identification and Phylogenetic Analysis of Newcastle Disease Virus Isolated from Duck Flocks

作　　者：孙杰[1] 刁有祥[1] 李建侠[1] 吴焕荣[1] 颜贇[1] 杨建朋[1] 李瑶瑶[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018

出　　处：《畜牧兽医学报》2010年第8期1054-1060,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：山东省科技攻关项目(2007GG30009001)

摘　　要：为调查山东省鸭群中新城疫病毒(NDV)的流行情况,对2007—2009年山东不同地区发病鸭群进行了NDV的分离鉴定,并对分离的10株鸭源NDV进行了生物学特性和遗传特性研究。结果显示:10株鸭源NDV的致病指数MDT和ICPI测定结果分别为50.4～60.0和1.66～1.78,表明10株分离株均属强毒株。致病性试验显示,3株代表毒株对不同日龄雏鸭均具有较强的致病性,发病率和死亡率分别达70%～100%和20%～70%。对F基因序列分析表明,相对于基因Ⅰ～Ⅵ型NDV,10株鸭源NDVF蛋白存在较大变异,这些变异主要集中在1—30、101—124、479—494和509—516 aa处,且在52、71、176、272、314、402和489位出现了与近几年流行毒株一致的氨基酸替代。核苷酸同源性分析结果表明,10株鸭源NDV之间同源性在95.8%～99.9%,与2000年以来国内外分离的基因Ⅶ型NDV同源性较高,在95.7%～99.8%,与传统LaSota、F48E9同源性较低,为83.8%～90.9%。遗传进化分析结果显示分离毒株均属于基因Ⅶd型。此外,10株鸭源NDV还具有大多数鹅源NDV所特有的973位和1 249位RsaⅠ位点。上述结果表明基因Ⅶd型NDV是造成我国鸭群近年来发生新城疫的主要病原,鸭源NDV很可能源于早期的鹅源NDV。To explore the epidemiological understanding of Newcastle disease virus（NDV） in ducks,10 NDVs isolated from outbreaks in duck flocks in Shandong Province during 2007-2009 were characterized both biologically and genetically.All 10 strains were velogenic strains based on their MDT（50.4-60.0） and ICPI（1.66-1.78）.Ducklings challenged with the strains showed visible clinical symptoms and autopsy changes,and the morbidity and mortality were up to 70%-100% and 20%-70%,respectively.Compared with genotype Ⅰ-Ⅵ NDV,great variation were occurred,especially at 1-30aa,101-124aa,479-494aa and 509-516aa of F protein of the 10 isolates,which displayed the same characteristic amino acid substitutions at position 52,71,176,272,314,402 and 489 with NDV strains obtained in recent years.Nucleotide analysis revealed that the homology among the 10 isolates was 95.8%-99.9%,and shared 95.7%-99.8% identities with genotype Ⅶ NDV since 2000,but only 83.8%-90.9% identities with LaSota and F48E9.Phylogenetic analysis showed that all the 10 isolates were of the genotype Ⅶd virus and had two RsaⅠ sites at position 973 and 1 249 bp which were characteristic to most strains of goose origin.The results indicated that the strains of genotype Ⅶd NDV had been the major pathogen responsible for the most epizootic ND outbreaks in duck flocks in China,and NDV isolated from ducks was likely originated from that of goose flocks.

关 键 词：鸭 新城疫 F基因 遗传特性 

分 类 号：S852.659.5[农业科学—基础兽医学]