微量元素浓度对3个籼稻品种愈伤组织褐化和分化的影响  

Effects of Microelement Concentrations on Callus Browning and Seedlings Differentiation of Three indica Rice Cultivars

作　　者：周凤[1,2] 于卉[1] 葛占宇[1] 周芝辉[1] 邓燕燕[1] 付永彩[1] 

机构地区：[1]中国农业大学植物遗传育种系,国家农业野生植物鉴定评价中心(水稻),农业部农作物基因组学与遗传改良重点开放实验室,北京市作物遗传改良重点实验室,北京100193 [2]山西大同大学农学与生命科学学院,大同037009

出　　处：《农业生物技术学报》2010年第4期702-706,共5页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(No.30771319);国家重大科技专项(2008ZX08001-004)共同资助

摘　　要：选用生产上应用的3个优良籼稻(Oryza sativa ssp.indica)品种桂朝2号、特青和9311的成熟胚为材料,以CC为基本培养基,经过愈伤组织诱导、两次继代、预分化、分化培养,研究培养基中不同微量元素浓度对3个籼稻品种成熟胚愈伤组织褐化和分化的影响。结果表明,在预分化和分化阶段,桂朝2号添加5×CC微量元素,9311添加3×CC微量元素可以明显抑制愈伤组织褐化,提高愈伤组织分化频率,而特青对微量元素浓度反应不敏感,研究结果为籼稻遗传改良和籼稻转基因研究提供良好的组织培养技术参考。The effects of different microelement concentrations in the culture medium on browning and differentiation of callus derived from mature embryos of three elite indica rice cultivars,Guichao2,Teqing and 9311 were investigated through the callus induction,two-time subculture,pre-differentiation and differentiation in this study.The results suggested that in the culture medium of pre-differentiation and differentiation,callus browning was inhibited and callus greening frequency was improved when Guichao2 callus was cultured with 5× CC microelement content and 9311 callus was cultured with 3×CC microelement content,but no effect for Teqing callus when increasing the microelement content.This study provides a new insight into indica rice genetic improvement and transformation.

关 键 词：籼稻 成熟胚 愈伤组织褐化 分化 

分 类 号：S567.239[农业科学—中草药栽培]